Molecular typing of human herpesvirus 8 among HIV positive in comparison to HIV‐negative individuals in Iran

• Published in: Journal of medical virology Vol. 89; no. 4; pp. 703 - 709
• Main Authors: Varmazyar, Sajad, Shoja, Zabihollah, Kakavand‐Ghalehnoei, Rezvan, Shahmahmoodi, Shohreh, Marashi, Sayed Mahdi, Jalilvand, Somayeh
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.04.2017
• Subjects: Adult; Aged; Aged, 80 and over; Female; Genotype; Genotype & phenotype; Genotyping Techniques; Herpes viruses; Herpesviridae; Herpesviridae Infections - epidemiology; Herpesviridae Infections - virology; Herpesvirus 8, Human - classification; Herpesvirus 8, Human - genetics; Herpesvirus 8, Human - isolation & purification; herpesvirus type 8; HIV; HIV Infections - complications; HIV patients; Human immunodeficiency virus; Humans; Iran; Iran - epidemiology; Kaposi's sarcoma; Lentivirus; Male; Middle Aged; Molecular Epidemiology; Molecular Typing; ORF K1; Pathogenesis; Polymerase Chain Reaction; Retroviridae; Virology; Iran; ORF K1; HIV patients; Iran; Kaposi's sarcoma; herpesvirus type 8
• ISSN: 0146-6615; 1096-9071; 1096-9071
• DOI: 10.1002/jmv.24644

It is still unclear whether different HHV‐8 genotypes may have different pathogenic and tumorigenic properties associated with a diverse rate of disease progression. In some areas where genotype C was found to be prominent among classic KS patients, genotype A was shown to be more frequent among AIDS‐associated KS patients. Genotype C was previously reported to be widespread in Iran, with genotype A being less frequent among patients with classic KS although no data are available with regards to the HHV‐8 genotyping among Iranian HIV‐infected patients. In order to analyze HHV‐8 genotypes (ORF K1), six HIV‐infected patients (with or without KS), and 22 HIV‐negative subjects (classic/iatrogenic KS patients and IVDUs) were investigated using nested PCR. Genotype A was detected more frequently among HIV‐infected patients with or without KS (three out of six) whereas genotype C was found more common among HIV‐negative subjects including classic/iatrogenic KS patients and IVDSs (21 out of 22), and this difference was statistically significant (P = 0.044). In conclusion, our data further support the dominancy of HHV‐8 genotype C in Iranian general population. Moreover, genotype A was more common among HIV‐infected patients with or without KS. J. Med. Virol. 89:703–709, 2017. © 2016 Wiley Periodicals, Inc.