TNF-α and leptin in experimental liver fibrosis models induced by carbon tetrachloride and by common bile duct ligation

• Published in: Cell biochemistry and function Vol. 22; no. 6; pp. 359 - 363
• Main Authors: Bahçecıoğlu, İbrahim Halil, Yalniz, Mehmet, Ataseven, Hüseyin, Bülbüller, Nurullah, Keçecı, Muzaffer, Demırdağ, Kutbeddin, Özercan, İbrahim, Üstündağ, Bilal
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.11.2004
• Subjects: Animals; Carbon Tetrachloride; Common Bile Duct - surgery; common bile duct ligation; Disease Models, Animal; leptin; Leptin - blood; Ligation; Liver Cirrhosis, Experimental - blood; Liver Cirrhosis, Experimental - chemically induced; Liver Cirrhosis, Experimental - etiology; liver fibrosis; Male; Rats; TNF-α; Tumor Necrosis Factor-alpha - analysis
• ISSN: 0263-6484; 1099-0844
• DOI: 10.1002/cbf.1114

In this study we investigated TNF‐α and leptin levels in two different liver fibrosis models induced by carbon tetrachloride (CCl4) and common bile duct ligation (CBDL). A total of 36 male rats of Albino‐Wistar strain were allocated to three groups. One of the groups was the control. The second group received 0.15 ml 100 g−1 CCl4 subcutaneously for 6 weeks, 3 days per week. The third group underwent common bile duct ligation (CBDL) and was monitored for 4 weeks. Histopathological investigation included fibrosis, steatosis and inflammation. Serum IL‐6 and TNF‐α levels were analysed by ELISA methods and leptin was analysed by RIA. Fibrosis and steatosis increased significantly in the CCl4 group in comparison with the CBDL group (p < 0.01; p < 0.001). Leptin and TNF‐α levels in CCl4 group were higher than those in the CBDL and control groups (p < 0.05). TNF‐α and leptin levels were not related to each another in either the CCl4 group or the CBDL group (r=0.22, p > 0.05; r=0.19, p > 0.05). The IL‐6 level was higher in the CCl4 group in relation to severity of inflammation (p < 0.05). TNF‐α and leptin levels were higher in animals with liver fibrosis induced by CCl4, than they were in those whose liver fibrosis was induced by common bile duct ligation. Leptin and TNF‐α may be less effective on the development of liver fibrosis in the group which underwent common bile duct ligation. Copyright © 2004 John Wiley & Sons, Ltd.