Fructose-1,6-diphosphate attenuates acute lung injury induced by ischemia-reperfusion in rats
To determine whether fructose-1,6-diphosphate (FDP) pretreatment can attenuate acute lung injury induced by ischemia-reperfusion in our isolated lung model in rats. Randomized, controlled study. Animal care facility procedure room. Twenty-four adult male Sprague-Dawley rats each weighing 250-350 g....
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Published in | Critical care medicine Vol. 30; no. 7; p. 1605 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
01.07.2002
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Subjects | |
Online Access | Get more information |
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Summary: | To determine whether fructose-1,6-diphosphate (FDP) pretreatment can attenuate acute lung injury induced by ischemia-reperfusion in our isolated lung model in rats.
Randomized, controlled study.
Animal care facility procedure room.
Twenty-four adult male Sprague-Dawley rats each weighing 250-350 g.
Typical acute lung injury in rats was induced successfully by 10 mins of hypoxia followed by 75 mins of ischemia and 50 mins of reperfusion. Ischemia-reperfusion significantly increased microvascular permeability as measured by the capillary filtration coefficient, lung weight gain, lung weight to body weight ratio, pulmonary arterial pressure, and protein concentration of bronchoalveolar lav-age fluid.
Pretreatment with FDP significantly attenuated the acute lung injury induced by ischemia-reperfusion as shown by a significant decrease in all of the assessed variables (p <.05 p <.001). The protective effect of FDP was nearly undetectable when promazine (an ecto-adenosine 5-triphosphatase inhibitor) was added before FDP pretreatment.
Pretreatment with FDP significantly ameliorates acute lung injury induced by ischemia-reperfusion in rats. |
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ISSN: | 0090-3493 |
DOI: | 10.1097/00003246-200207000-00034 |