Fructose-1,6-diphosphate attenuates acute lung injury induced by ischemia-reperfusion in rats

• Published in: Critical care medicine Vol. 30; no. 7; p. 1605
• Main Authors: Chu, Shi-Jye, Chang, Deh-Ming, Wang, David, Chen, Ying-Hsin, Hsu, Chin-Wang, Hsu, Kang
• Format: Journal Article
• Language: English
• Published: United States 01.07.2002
• Subjects: Animals; Blood Pressure; Capillary Permeability; Fructosediphosphates - antagonists & inhibitors; Fructosediphosphates - therapeutic use; Immunologic Factors - therapeutic use; In Vitro Techniques; Male; Organ Size; Promazine - pharmacology; Pulmonary Artery; Rats; Rats, Sprague-Dawley; Reperfusion Injury; Respiratory Distress Syndrome, Adult - drug therapy; Respiratory Distress Syndrome, Adult - etiology; Respiratory Distress Syndrome, Adult - pathology
• ISSN: 0090-3493
• DOI: 10.1097/00003246-200207000-00034

To determine whether fructose-1,6-diphosphate (FDP) pretreatment can attenuate acute lung injury induced by ischemia-reperfusion in our isolated lung model in rats. Randomized, controlled study. Animal care facility procedure room. Twenty-four adult male Sprague-Dawley rats each weighing 250-350 g. Typical acute lung injury in rats was induced successfully by 10 mins of hypoxia followed by 75 mins of ischemia and 50 mins of reperfusion. Ischemia-reperfusion significantly increased microvascular permeability as measured by the capillary filtration coefficient, lung weight gain, lung weight to body weight ratio, pulmonary arterial pressure, and protein concentration of bronchoalveolar lav-age fluid. Pretreatment with FDP significantly attenuated the acute lung injury induced by ischemia-reperfusion as shown by a significant decrease in all of the assessed variables (p <.05 p <.001). The protective effect of FDP was nearly undetectable when promazine (an ecto-adenosine 5-triphosphatase inhibitor) was added before FDP pretreatment. Pretreatment with FDP significantly ameliorates acute lung injury induced by ischemia-reperfusion in rats.