A preliminary study of the dental implant therapy—initial osteogenesis of Human Mesenchymal Stem (HMS0014) cells on titanium discs with different surface modifications

• Published in: Okajimas Folia Anatomica Japonica Vol. 88; no. 4; pp. 133 - 140
• Main Authors: IWAI, Yasutomo, MATSUDA, Yoshifumi, NAKATSUKA, Michiko, MIKAMI, Yutaka, KUMABE, Shunji
• Format: Journal Article
• Language: English
• Published: Japan Editorial Board of Okajimas Folia Anatomica Japonica 01.02.2012
• Subjects: Alkaline Phosphatase - metabolism; Calcium - metabolism; Cell Adhesion; Cell Proliferation; Cells, Cultured; Dental Implants; GBR; HMS0014 cell; Humans; in vitro; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - metabolism; Mesenchymal Stromal Cells - ultrastructure; Osseointegration; Osteoblasts - cytology; Osteoblasts - metabolism; Osteoblasts - ultrastructure; Osteocalcin - metabolism; Osteogenesis; Osteogenesis - physiology; peptide-based hydrogel scaffold; Surface Properties; Titanium
• ISSN: 0030-154X; 1881-1736
• DOI: 10.2535/ofaj.88.133

HMS0014 cells were GBR-engineered to proliferate and differentiate into mature osteoblast(Ob)-like cells, which initiated hard tissue matrix deposition in both monolayer and PuraMatrix 3-D cultures. Subsequently, the osteogenesis initiated with attachment/adhesion of HMS0014 cells on either Titanium (Ti) or Ti alloy discs modified with osteoconductive/ osteoinductive surface textures/substrates (e.g., Disc-AO, Disc-HA, Disc-SPI) was histologically assessed. The results obtained were as follows: 1) The HMS0014 cells actively proliferated and differentiated into mature Obs to initiate mineralisation of the ECM since day 1 in both monolayer and 3-D cultures; mineralization was prominently progressed between day 7 and day 14 of cultures. 2) The SEM of 60-minute(min)s specimens demonstrated a loose distribution of proliferating spherical-to-polygonal (10 to 40 μm in diameter, avg.) cells with a bulging cell body sending out many minute filopodia and some lamellipodia to attach with the substrate in the concavities. 3) In the 180-min specimens, the cultured HMS0014 cells actively proliferated and spread into flat, large polygonal cells with prominent lamellipodia and dendritic filopodia (30 μm × 90 μm to 100 μm × 200 μm, approx.) to employ cell-to-substrate and intercellular attachments. 4) On the other hand, the present immunohistochemistry of the attached HMS0014 cells demonstrated the co-expression of F-actin (actin filaments of the cytoskeleton) and CD51 (αV integrin) in both the 60-min and 180-min specimens. We concluded that the present GBR method enhanced HMS0014 cells to initiate an osteogenesis process with a direct bone-to-substratum contact on Ti discs which were subject to different surface modifications.