Fabrication and Properties of Hydrogel Dressings Based on Genipin Crosslinked Chondroitin Sulfate and Chitosan

• Published in: Polymers Vol. 16; no. 20; p. 2876
• Main Authors: Wang, Ling, Ding, Xiaoyue, He, Xiaorui, Tian, Ning, Ding, Peng, Guo, Wei, Okoro, Oseweuba Valentine, Sun, Yanfang, Jiang, Guohua, Liu, Zhenzhong, Shavandi, Armin, Nie, Lei
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11.10.2024; MDPI
• Subjects: Acids; Ammonium chloride; Ammonium compounds; Ammonium paratungstate; Biocompatibility; Care and treatment; Chitosan; Chloride; Chondroitin; Chondroitin sulfate; Crosslinking; Dopamine; E coli; Escherichia coli; Fluorescence; Fourier transforms; Genipin; Hemodialysis; hydrogel; Hydrogels; Infrared analysis; Injectability; interpenetrated network; Interpenetrating networks; Loss modulus; Magnetic properties; Mechanical properties; NMR; Nuclear magnetic resonance; Nuclear magnetic resonance spectroscopy; Rheological properties; Rheology; Scanning electron microscopy; Storage modulus; Sulfates; Water; wound dressings; Wound healing; Wounds and injuries; China; United States > US; Japan; chitosan; hydrogel; chondroitin sulfate; genipin; wound dressings; interpenetrated network
• ISSN: 2073-4360; 2073-4360
• DOI: 10.3390/polym16202876

Multifunctional hydrogel dressings remain highly sought after for the promotion of skin wound regeneration. In the present study, multifunctional CHS-DA/HACC (CH) hydrogels with an interpenetrated network were constructed using hydroxypropyl trimethyl ammonium chloride modified chitosan (HACC) and dopamine-modified chondroitin sulfate (CHS-DA), using genipin as crosslinker. The synthesis of HACC and CHS-DA was effectively confirmed using Fourier transform infrared (FT-IR) analysis and H nuclear magnetic resonance ( H NMR) spectroscopy. The prepared CH hydrogels exhibited a network of interconnected pores within the microstructure. Furthermore, rheological testing demonstrated that CH hydrogels exhibited strong mechanical properties, stability, and injectability. Further characterization investigations showed that the CH hydrogels showed favorable self-healing and self-adhesion properties. It was also shown that increasing HACC concentration ratio was positively correlated with the antibacterial activity of CH hydrogels, as evidenced by their resistance to and . Additionally, Cell Counting Kit-8 (CCK-8) tests, fluorescent images, and a cell scratch assay demonstrated that CH hydrogels had good biocompatibility and cell migration ability. The multifunctional interpenetrated network hydrogels were shown to have good antibacterial properties, antioxidant properties, stable storage modulus and loss modulus, injectable properties, self-healing properties, and biocompatibility, highlighting their potential as wound dressings in wound healing applications.