Drynaria rhizome water extract alleviates high‑fat diet‑induced obesity in mice

• Published in: Molecular medicine reports Vol. 29; no. 2
• Main Authors: Gil, Tae-Young, Park, Junkyu, Park, Yea-Jin, Kim, Hyo-Jung, Cominguez, Divina C, An, Hyo-Jin
• Format: Journal Article
• Language: English
• Published: Greece Spandidos Publications 01.02.2024; Spandidos Publications UK Ltd
• Subjects: Adenosine kinase; Adipocytes; AMP; AMP-activated protein kinase; AMP-Activated Protein Kinases - metabolism; Animals; Anti-Obesity Agents - pharmacology; Antibodies; Biotechnology; Body fat; Body Weight; Bone diseases; Bone growth; Bone turnover; Cardiovascular disease; Care and treatment; Causes of; Chemical properties; Coronaviruses; Diet; Diet, High-Fat - adverse effects; DNA binding proteins; Ferns; Flavonoids; Food; Food intake; Fractures; Health aspects; Herbal medicine; High fat diet; Homeostasis; Insulin resistance; Kinases; Laboratory animals; Lipids; Lipogenesis; Liver; Materia medica, Vegetable; Medicine, Botanic; Medicine, Herbal; Metabolism; Mice; Mice, Inbred C57BL; Mice, Obese; Natural products; Obesity; Obesity - drug therapy; Obesity - etiology; Obesity - metabolism; Osteogenesis; Osteoporosis; Peroxisome Proliferator-Activated Receptors; Pharmaceutical industry; Plant extracts; Plant Extracts - pharmacology; Protein binding; Protein kinases; Proteins; Rhizome; Rhizomes; Sterol Regulatory Element Binding Protein 1 - genetics; Sterol Regulatory Element Binding Protein 1 - metabolism; Sterol regulatory element-binding protein; Transcription factors; Type 2 diabetes; Weight control; Western blotting; South Korea; Drynaria rhizome; sterol regulatory element binding protein‑1; peroxisome proliferator‑activated receptor‑γ; AMP‑activated protein kinase; high‑fat diet
• ISSN: 1791-2997; 1791-3004
• DOI: 10.3892/mmr.2023.13153

Drynaria rhizome is a herbal medicine used for strengthening bones and treating bone diseases in East Asia. Although obesity is considered to benefit bone formation, it has been revealed that visceral fat accumulation can promote osteoporosis. Given the complex relationship between bone metabolism and obesity, bone‑strengthening medicines should be evaluated while considering the effects of obesity. The present study investigated the effects of Drynaria rhizome extract (DRE) on high‑fat diet (HFD)‑induced obese mice. DRE was supplemented with the HFD. Body weight, food intake, the expression levels of lipogenesis transcription factors, including sterol regulatory element binding protein (SREBP)‑1, peroxisome proliferator‑activated receptor (PPAR)‑γ and adenosine monophosphate‑activated protein kinase (AMPK)‑α, and AMPK activation were evaluated. Mice fed DRE and a HFD exhibited reduced body weight without differences in food intake compared with those in the HFD group. Furthermore, DRE; upregulated AMPK‑α of epididymal one; down‑regulated SREBP‑1 and PPAR‑γ, as determined using western blotting and quantitative polymerase chain reaction, respectively. Decreased lipid accumulation were observed in both fat pad and liver of HFD‑fed mice, which were suppressed by DRE treatment. These results demonstrated the potential of DRE as a dietary natural product for strengthening bones and managing obesity.