Efficient biotinylation of methacryloyl-functionalized nonadherent cells for formation of cell microarrays

In order to obtain cell microarrays formed with human promyelocytic leukemia cells (HL-60), biotinylation of methacryloyl-functionalized HL-60 cells was performed via a thiol-ene reaction with thiol-terminated 4-arm poly(ethylene glycol) (PEG(4)10K-SH). Biotinylated HL-60 cells were selectively adhe...

Full description

Saved in:
Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 47; no. 37; pp. 10329 - 10331
Main Authors Iwasaki, Yasuhiko, Ota, Tatsuya
Format Journal Article
LanguageEnglish
Published England 01.01.2011
Subjects
Biotinylation
Cell Proliferation
Glycols
Hexosamines - chemistry
Hexosamines - metabolism
HL-60 Cells
Human
Humans
Leukemias
Polyethylene Glycols - chemistry
Sulfhydryl Compounds - chemistry
Tissue Array Analysis - methods
Viability
Online AccessGet full text

Cover

More Information
Summary:In order to obtain cell microarrays formed with human promyelocytic leukemia cells (HL-60), biotinylation of methacryloyl-functionalized HL-60 cells was performed via a thiol-ene reaction with thiol-terminated 4-arm poly(ethylene glycol) (PEG(4)10K-SH). Biotinylated HL-60 cells were selectively adhered onto an avidin-patterned surface with high viability.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:1359-7345
1364-548X
DOI:10.1039/c1cc12948a