Antibody responses of cows during an outbreak of neosporosis evaluated by indirect fluorescent antibody test and different enzyme-linked immunosorbent assays

• Published in: The Journal of parasitology Vol. 83; no. 6; p. 1063
• Main Authors: Dubrey, J.P. (USDA, ARS, Livestock and Poultry Sciences Institute, Beltsville, MD.), Jenkins, M.C, Adams, D.S, McAllister, M.M, Anderson-Sprecher, R, Baszler, T.V, Kwok, O.C.H, Lally, N.C, Bjorkman, C, Uggla, A
• Format: Journal Article
• Language: English
• Published: United States 01.12.1997
• Subjects: ABORTION; Abortion, Veterinary - epidemiology; Abortion, Veterinary - immunology; Animals; ANTIBODIES; Antibodies, Protozoan - biosynthesis; Antibodies, Protozoan - blood; Antigens, Protozoan - immunology; BLOOD SERUM; CALIFORNIA; California - epidemiology; Cattle; Cattle Diseases - epidemiology; Cattle Diseases - immunology; Coccidiosis - epidemiology; Coccidiosis - immunology; Coccidiosis - veterinary; DAIRY COWS; DIAGNOSIS; Disease Outbreaks - statistics & numerical data; Disease Outbreaks - veterinary; ELISA; Enzyme-Linked Immunosorbent Assay - statistics & numerical data; Female; Fluorescent Antibody Technique, Indirect - statistics & numerical data; IMMUNOFLUORESCENCE; Neospora - immunology; NEOSPORA CANINUM; Pregnancy; Pregnancy Complications, Parasitic - epidemiology; Pregnancy Complications, Parasitic - immunology; Pregnancy Complications, Parasitic - veterinary; Probability; PROTOZOAL INFECTIONS; Toxoplasma - immunology; California
• ISSN: 0022-3395; 1937-2345
• DOI: 10.2307/3284363

Serum samples from 70 (33 aborting and 37 non-aborting) dairy cows from a herd in California were analyzed for Neospora caninum antibodies in different laboratories by various serologic assays including enzyme-linked immunosorbent assay (ELISA) with recombinant antigens (Nc4.1 and Nc14.1), kinetic ELISA, whole tachyzoite lysate ELISA, immunostimulating complex (iscom) ELISA, antigen capture competitive inhibition ELISA, and by the indirect fluorescent antibody test. Eighteen percent of pregnant cows in this herd had aborted within 2 mo of the index case. All 70 cows had antibodies to N. caninum by at least 1 of the tests. Antibody levels to N. caninum in aborting cows as a group were higher than in nonaborting cows. However, it was concluded that no serological test could be used to establish definitively that N. caninum caused the abortion in an individual cow.