Papiliotrema siamense f.a., sp. nov., a yeast species isolated from plant leaves

Two strains representing a novel species were isolated from the external surface of a sugar cane leaf (DMKU-SP85T) and tissue of a rice leaf (DMKU-RE97) collected in Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and sequence analysis of the D...

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Published inInternational journal of systematic and evolutionary microbiology Vol. 64; no. Pt 9; pp. 3058 - 3062
Main Authors Surussawadee, Janjira, Khunnamwong, Pannida, Srisuk, Nantana, Limtong, Savitree
Format Journal Article
LanguageEnglish
Published England International Union of Microbiological Societies 01.09.2014
Subjects
Basidiomycota - classification
Basidiomycota - genetics
Basidiomycota - isolation & purification
chemotaxonomy
DNA, Fungal - genetics
DNA, Ribosomal Spacer - genetics
genes
Genes, rRNA
internal transcribed spacers
leaves
Molecular Sequence Data
Mycological Typing Techniques
nucleotides
Oryza - microbiology
Phylogeny
Plant Leaves - microbiology
ribosomal RNA
rice
Saccharum - microbiology
sequence analysis
Sequence Analysis, DNA
sexual reproduction
sugarcane
Thailand
yeasts
Thailand
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Summary:Two strains representing a novel species were isolated from the external surface of a sugar cane leaf (DMKU-SP85T) and tissue of a rice leaf (DMKU-RE97) collected in Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, the two strains were determined to represent a novel species of the genus Papiliotrema although sexual reproduction was not observed. The sequences of the D1/D2 region of the LSU rRNA gene and ITS region of the two strains were identical, but differed from those of the type strain of Cryptococcus nemorosus by 0.6 % nucleotide substitutions (four nucleotide substitutions out of 597 nucleotides) in the D1/D2 region of the LSU rRNA gene and 1.8 % nucleotide substitutions (nine nucleotide substitutions out of 499 nucleotides) in the ITS region. The name Papiliotrema siamense f.a., sp. nov. is proposed. The type strain is DMKU-SP85T( = BCC 69499T = CBS 13330T).
Bibliography:http://dx.doi.org/10.1099/ijs.0.065607-0
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ISSN:1466-5034
1466-5026
1466-5034
DOI:10.1099/ijs.0.065607-0