Helicobacter pylori infection influences expression of genes related to angiogenesis and invasion in human gastric carcinoma cells

• Published in: Biochemical and biophysical research communications Vol. 311; no. 4; pp. 809 - 814
• Main Authors: Kitadai, Yasuhiko, Sasaki, Atsunori, Ito, Masanori, Tanaka, Shinji, Oue, Naohide, Yasui, Wataru, Aihara, Miki, Imagawa, Kenichi, Haruma, Ken, Chayama, Kazuaki
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 28.11.2003
• Subjects: Adenocarcinoma - genetics; Adenocarcinoma - metabolism; Adenocarcinoma - pathology; Angiogenesis; Angiogenesis Inducing Agents - metabolism; Carcinoma, Adenosquamous - genetics; Carcinoma, Adenosquamous - metabolism; Carcinoma, Adenosquamous - pathology; cDNA microarray; Cell Line, Tumor; Collagenases - biosynthesis; Endopeptidases - genetics; Gastric carcinoma; Gene Expression Regulation, Bacterial - genetics; Helicobacter Infections - complications; Helicobacter Infections - genetics; Helicobacter pylori; Helicobacter pylori - genetics; Humans; Invasion; Neovascularization, Pathologic - genetics; Stomach Neoplasms - etiology; Stomach Neoplasms - genetics; Stomach Neoplasms - metabolism; Stomach Neoplasms - pathology; Angiogenesis; Gastric carcinoma; cDNA microarray; Helicobacter pylori; Invasion
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2003.10.077

Infection with Helicobacter pylori ( H. pylori) is considered a risk factor for gastric carcinoma. The purpose of this study was to clarify whether H. pylori infection plays a role in progression of gastric carcinoma. We examined the expression of genes encoding angiogenic factors and proteases by human gastric carcinoma cell lines (MKN-1 and TMK-1) co-cultured with or without H. pylori by cDNA microarray analysis. Co-culture with H. pylori increased expression of mRNAs encoding interleukin (IL)-8, vascular endothelial growth factor (VEGF), angiogenin, urokinase-type plasminogen activator (uPA), and metalloproteinase (MMP)-9 by gastric carcinoma cells. Up-regulation of these genes at the mRNA and protein levels was confirmed by Northern blot analysis, semi-quantitative RT-PCR analysis, and ELISA. In vitro angiogenic and collagenase activities of conditioned medium from the gastric carcinoma cells were also stimulated by co-culture with H. pylori. These results indicate that H. pylori infection may regulate angiogenesis and invasion of human gastric carcinoma.