Novel Polyurethane Scaffolds Containing Sucrose Crosslinker for Dental Application

• Published in: International journal of molecular sciences Vol. 23; no. 14; p. 7904
• Main Authors: Kordován, Marcell Árpád, Hegedűs, Csaba, Czifrák, Katalin, Lakatos, Csilla, Kálmán-Szabó, Ibolya, Daróczi, Lajos, Zsuga, Miklós, Kéki, Sándor
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 18.07.2022; MDPI
• Subjects: Biocompatibility; biological testing; Carbohydrates; Crosslinking; Dental materials; Dental pulp; Dentistry; Differential scanning calorimetry; Experiments; Hexamethylene diisocyanate; Hydrophobicity; Infrared spectroscopy; Lactic acid; Magnetic resonance spectroscopy; Mass spectrometry; Mass spectroscopy; Mechanical properties; mechanical testing; Morphology; NMR; NMR spectroscopy; Nuclear magnetic resonance; poly(ε-caprolactone); Polycaprolactone; Polycondensation reactions; Polyethylene glycol; Polylactic acid; polylactic acid diol; Polymers; Polyurethane; Polyurethane resins; Prepolymers; Spectrum analysis; Stem cells; Sucrose; Tensile strength; Tensile tests; Thermomechanical properties; Tissue engineering; Toxicity
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms23147904

In this paper, the synthesis, characterization, and properties of crosslinked poly(ε-caprolactone)-based polyurethanes as potential tissue replacement materials are reported. The polyurethane prepolymers were prepared from poly(ε-caprolactone)diol (PCD), polyethylene glycol (PEG)/polylactic acid diol (PLAD), and 1,6-hexamethylene diisocyanate (HDI). In these segmented polyurethanes, the role of PEG/PLAD was to tune the hydrophobic/hydrophilic character of the resulting polymer while sucrose served as a crosslinking agent. PLAD was synthesized by the polycondensation reaction of D,L-lactic acid and investigated by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and nuclear magnetic resonance spectroscopy (NMR). The crosslinked polyurethane samples (SUPURs) obtained were characterized by attenuated total reflectance Fourier-transform infrared spectroscopy (AT-FT-IR), swelling, and mechanical (uniaxial tensile tests) experiments. The thermo and thermomechanical behavior were studied by differential scanning calorimetry (DSC) and dynamical mechanical analysis (DMA). The viability of dental pulp stem cells was investigated in the case of polyurethanes composed of fully biocompatible elements. In our studies, none of our polymers showed toxicity to stem cells (DPSCs).