Micropropagation of Panax notoginseng by somatic embryogenesis and RAPD analysis of regenerated plantlets

• Published in: Plant cell reports Vol. 16; no. 7; pp. 450 - 453
• Main Authors: Shoyama, Y, Zhu, X.X, Nakai, R, Shiraishi, S, Kohda, H
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.04.1997
• Subjects: Biological and medical sciences; Biotechnology; culture media; dna amplification; embryo (plant); Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; genetic techniques and protocols; In vitro propagation: entire plant regeneration from tissues and cell cultures; methodology; Methods. Procedures. Technologies; micropropagation; Panax; Plant cells and fungal cells; random amplified polymorphic DNA technique; regenerative ability; somatic embryogenesis; Culture medium; Embryonic development; Purine derivatives; Callus; Vegetals; Auxin; Genetic marker; 1-Naphthaleneacetic acid; Tissue culture; Gibberellin; Micropropagation; Cytokinin; Somatic embryo; Medicinal plant; Regeneration; Random amplified polymorphic DNA; Dicotyledones; Angiospermae; Plant growth substance; Spermatophyta; Gibberellic acid; Clonal propagation; Araliaceae; Polymorphism
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s002990050258

Somatic embryogenesis was induced in callus tissues derived from young flower buds of Panax notoginseng via callus within 18 weeks of culture. The mature somatic embryos were germinated on half-strength Murashige and Skoog's (MS) medium supplemented with gibberellic acid A3(GA) and 6-benzyladenine (BA). The most suitable medium for optimal root formation proved to be MS medium supplemented with 1-naphthaleneacetic acid (NAA). Total DNA was extracted from the leaves of the regenerated plantlets of P. notoginseng. Analysis of random-amplified polymorphic DNA (RAPD) using 21 arbitrary oligonucleotide 10-mers, showed the genetic homogeneity of P. notoginseng. The amplification products were monomorphic for all of the plantlets of P. notoginseng regenerated by embryogenesis, suggesting that somatic embryogenesis can be used for clonal micropropagation of this plant.