Determination of Herpes Simplex Virus Type-Specific Antibodies by Solid-Phase RIA on Helix pomatia Lectin-Purified Antigens

• Published in: The Journal of infectious diseases Vol. 149; no. 6; pp. 964 - 972
• Main Authors: Suchánková, Alena, Hirsch, Ivan, Krěmárř, Martin, Vonka, Vladimír
• Format: Journal Article
• Language: English
• Published: United States The University of Chicago Press 01.06.1984; University of Chicago Press
• Subjects: Adult; Animals; Antibodies; Antibodies, Monoclonal - immunology; Antibodies, Viral - analysis; Antigens; Antigens, Viral - isolation & purification; Bacterial Proteins - immunology; Child; Child, Preschool; Cross Reactions; Female; Glycoproteins; Glycoproteins - immunology; Guinea Pigs; Herpes Genitalis - immunology; herpes simplex virus 1; herpes simplex virus 2; Human herpesvirus 1; Human herpesvirus 2; Humans; Immunization; Infections; Lectins; Male; Middle Aged; Monoclonal antibodies; Neutralization Tests; Original Articles; Rabbits; Radioimmunoassay; Reactivity; Sexual Behavior; Simplexvirus; Simplexvirus - classification; Simplexvirus - immunology; Viral Envelope Proteins; Viral Proteins - immunology; Viruses
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1093/infdis/149.6.964

Herpes simplex virus type 1 (HSV-1) and type 2 (HSY-2) antibodies in humans and animals were determined by a micro-solid-phase radioimmunoassay with Helix pomatia lectin/Sepharose 4B-purified antigens from HSY-l- and HSY-2-infected cells. A low but constant heterotypic reactivity was found with HSY-1 antigen, whereas HSY-2 antigen was type specific, as evidenced by (1) its reactivity with sera from HSY-2- but not with those from HSY-1-immunized animals, (2) its reactivity with monoclonal antibody to glycoprotein C but not with that to other HSY-2 glycoproteins, and (3) the negative results obtained in serum samples from 57 children, of whom 49 possessed HSY-1 neutralizing antibody. Antibody to HSY-2(anti-HSY-2) was detected in serum samples from 14 of 16 subjects treated for genital herpetic lesions. The prevalence of anti-HSY-2 in women did not differ in the 25–35- and 36–45-year-old age groups but did correlate with the number of sex partners, with only 10% of women reporting one sex partner and 33% of those reporting more than 10 sex partners having anti-HSY-2.