Bone-inducing activity of mature BMP-2b produced from a hybrid BMP-2a/2b precursor
The human osteoinductive proteins BMP-2a and BMP-2b have been cloned and expressed in mammalian cells. In order to improve expression levels we examined the role of the proregion in assembly and export. Use of the BMP-2a proregion combined with the mature region of BMP-2b leads to dramatically impro...
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Published in | Molecular endocrinology (Baltimore, Md.) Vol. 5; no. 1; pp. 149 - 155 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Bethesda, MD
Endocrine Society
1991
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Subjects | |
Online Access | Get full text |
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Summary: | The human osteoinductive proteins BMP-2a and BMP-2b have been cloned and expressed in mammalian cells. In order to improve expression levels we examined the role of the proregion in assembly and export. Use of the BMP-2a proregion combined with the mature region of BMP-2b leads to dramatically improved expression of mature BMP-2b. Mature BMP-2b has been purified to near homogeneity from the BMP-2a/2b hybrid, and its structural properties and biological activity determined. Recombinant mature BMP-2b homodimer elicits bone formation in vivo. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0888-8809 1944-9917 |
DOI: | 10.1210/mend-5-1-149 |