Bone-inducing activity of mature BMP-2b produced from a hybrid BMP-2a/2b precursor

• Published in: Molecular endocrinology (Baltimore, Md.) Vol. 5; no. 1; pp. 149 - 155
• Main Authors: GELNN HAMMONDS, R. JR, SCHWALL, R, DUDLEY, A, BERKEMEIER, L, LAI, C, LEE, J, CUNNINGHAM, N, REDDI, A. H, WOOD, W. I, MASON, A. J
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Endocrine Society 1991
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Bone and Bones - drug effects; Bone Development - drug effects; Bone Morphogenetic Proteins; Cell Line; Cloning, Molecular; Fundamental and applied biological sciences. Psychology; Gene Expression; Humans; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Protein Precursors - genetics; Proteins - chemistry; Proteins - genetics; Proteins - pharmacology; Rats; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - pharmacology; Transfection; Human; Vertebrata; Mammalia; Molecular processing; Rat; Rodentia; Bone; Gene expression; Hybrid gene; Osteogenesis
• ISSN: 0888-8809; 1944-9917
• DOI: 10.1210/mend-5-1-149

The human osteoinductive proteins BMP-2a and BMP-2b have been cloned and expressed in mammalian cells. In order to improve expression levels we examined the role of the proregion in assembly and export. Use of the BMP-2a proregion combined with the mature region of BMP-2b leads to dramatically improved expression of mature BMP-2b. Mature BMP-2b has been purified to near homogeneity from the BMP-2a/2b hybrid, and its structural properties and biological activity determined. Recombinant mature BMP-2b homodimer elicits bone formation in vivo.