Prevotella histicola sp. nov., isolated from the human oral cavity

• Published in: International journal of systematic and evolutionary microbiology Vol. 58; no. 8; pp. 1788 - 1791
• Main Authors: Downes, Julia, Hooper, Samuel J, Wilson, Melanie J, Wade, William G
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.08.2008; Society for General Microbiology
• Subjects: Acetic Acid - metabolism; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; Carcinoma, Squamous Cell - microbiology; DNA, Bacterial - analysis; DNA, Ribosomal - analysis; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Genotype; Humans; Microbiology; Molecular Sequence Data; Mouth Mucosa - microbiology; Mouth Neoplasms - microbiology; Nucleic Acid Hybridization; Phenotype; Phylogeny; Prevotella; Prevotella - classification; Prevotella - genetics; Prevotella - isolation & purification; Prevotella - metabolism; Prevotella melaninogenica; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Species Specificity; Succinic Acid - metabolism; Systematics; Human; Oral cavity; Taxonomy; Bacteria; New species; Prevotella; Bacteroidaceae
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.65656-0

1 King's College London Dental Institute at Guy's, King's College and St Thomas' Hospitals, Infection Research Group, London SE1 9RT, UK 2 Cardiff University School of Dentistry, Heath Park, Cardiff CF14 4XY, UK Correspondence William G. Wade william.wade{at}kcl.ac.uk Three strains of anaerobic, variably pigmenting, Gram-negative bacilli isolated from human oral mucosal tissue were subjected to a comprehensive range of phenotypic and genotypic tests and were found to comprise a homogeneous group. 16S rRNA gene sequence analysis and DNA–DNA hybridization revealed that the strains constituted a novel group within the genus Prevotella , being most closely related to Prevotella melaninogenica and Prevotella veroralis . A novel species, Prevotella histicola sp. nov., is proposed to accommodate these strains. Prevotella histicola is saccharolytic and produces acetic acid and succinic acid as major end products of fermentation and trace to minor amounts of isovaleric acid and lactic acid. The G+C content of the DNA of the type strain is 43 mol%. The type strain of Prevotella histicola is T05-04 T (=DSM 19854 T =CCUG 55407 T ). The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains T05-04 T , N12-20 and N19-30 are EU126661 , EU126662 and EU130901 , respectively. An extended neighbour-joining phylogenetic tree and a summary of the characteristics of the novel species are available as supplementary material with the online version of this paper.