Isolation and characterization of Viviparous-1 haplotypes in wheat related species
Pre-harvest sprouting (PHS) resistance is one of the most important traits in wheat breeding. Characterization of Viviparous - 1 ( Vp - 1 ) haplotypes in wheat related species will further our understanding of the role of Vp - 1 in PHS resistance of bread wheats. The present paper reported Vp - 1 ha...
Saved in:
Published in | Euphytica Vol. 188; no. 1; pp. 71 - 84 |
---|---|
Main Authors | , , , , |
Format | Journal Article Conference Proceeding |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.11.2012
Springer Springer Nature B.V |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Pre-harvest sprouting (PHS) resistance is one of the most important traits in wheat breeding. Characterization of
Viviparous
-
1
(
Vp
-
1
) haplotypes in wheat related species will further our understanding of the role of
Vp
-
1
in PHS resistance of bread wheats. The present paper reported
Vp
-
1
haplotype analyzes of 77 accessions of wheat related species including
T. monococcum
and
T.
boeoticum
(A
m
A
m
),
T.
durum
(AABB),
T. dicoccoides
(AABB) and
Ae. tauschii
(DD). A total of 11 novel
Vp
-
1
haplotypes were identified in these species including three in
T. monococcum
which were designated as
TmVp
-
1A1
,
TmVp
-
1A2
,
TmVp
-
1A3
, three in
T.
boeoticum
, designated as
TbVp
-
1A1
,
TbVp
-
1A2
and
TbVp
-
1A3
, two in
T.
durum
, designated as
TduVp
-
1B1
and
TduVp
-
1B2
, and three in
Ae. tauschii
designated as
AetVp
-
1D1
,
AetVp
-
1D2
and
AetVp
-
1D3
, respectively. Among these haplotypes explored,
TduVp
-
1B1
was identical to
TaVp
-
1Be
which was detected in a PHS resistant Chinese landrace. Semi-quantitative RT-PCR analysis demonstrated that the presence of alternatively spliced transcripts of the
Vp
-
1
homologues in these wheat related species. The level of correctly spliced transcripts varies among the haplotypes, and was correlated with the degree of ABA responsiveness during seed germination. It appeared that
Vp
-
1
mis-splicing and some indel variations in bread wheats originated from its progenitors and were retained during polyploidization. Moreover, haplotypes with better
Vp
-
1
splicing such as
TbVp
-
1A2
of
T.
boeoticum
and
TduVp
-
1B1
of
T.
durum
species might be valuable in breeding PHS tolerant wheat. |
---|---|
ISSN: | 0014-2336 1573-5060 |
DOI: | 10.1007/s10681-012-0659-3 |