Sphingosine Kinase Type 1 Promotes Estrogen-Dependent Tumorigenesis of Breast Cancer MCF-7 Cells

• Published in: Experimental cell research Vol. 281; no. 1; pp. 115 - 127
• Main Authors: Nava, Victor E., Hobson, John Peyton, Murthy, Shvetha, Milstien, Sheldon, Spiegel, Sarah
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.11.2002
• Subjects: Adenocarcinoma - enzymology; Adenocarcinoma - pathology; angiogenesis; Animals; Antineoplastic Agents - pharmacology; breast cancer; Breast Neoplasms - enzymology; Breast Neoplasms - pathology; Estrogens - metabolism; Humans; Lysophospholipids; Mammary Neoplasms, Experimental - enzymology; Mammary Neoplasms, Experimental - pathology; MCF-7 cells; Mice; Mice, Inbred BALB C; Mice, Nude; Mitogen-Activated Protein Kinase 3; Mitogen-Activated Protein Kinases - metabolism; Neoplasms, Hormone-Dependent - enzymology; Neoplasms, Hormone-Dependent - pathology; nude mice; Phosphotransferases (Alcohol Group Acceptor) - physiology; Receptors, Cell Surface - metabolism; Receptors, Estrogen - metabolism; Receptors, G-Protein-Coupled; Receptors, Lysophospholipid; Sphingosine - analogs & derivatives; Sphingosine - metabolism; sphingosine kinase; sphingosine-1-phosphate; Tumor Cells, Cultured - drug effects; Tumor Cells, Cultured - enzymology; tumorigenesis; sphingosine kinase; angiogenesis; breast cancer; MCF-7 cells; nude mice; sphingosine-1-phosphate; tumorigenesis
• ISSN: 0014-4827; 1090-2422
• DOI: 10.1006/excr.2002.5658

The sphingolipid metabolite, sphingosine-1-phosphate (S1P), formed by phosphorylation of sphingosine, has been implicated in cell growth, suppression of apoptosis, and angiogenesis. In this study, we have examined the contribution of intracellular S1P to tumorigenesis of breast adenocarcinoma MCF-7 cells. Enforced expression of sphingosine kinase type 1 (SPHK1) increased S1P levels and blocked MCF-7 cell death induced by anti-cancer drugs, sphingosine, and TNF-α. SPHK1 also conferred a growth advantage, as determined by proliferation and growth in soft agar, which was estrogen dependent. While both ERK and Akt have been implicated in MCF-7 cell growth, SPHK1 stimulated ERK1/2 but had no effect on Akt. Surprisingly, parental growth of MCF-7 cells was only weakly stimulated by S1P or dihydro-S1P, ligands for the S1P receptors which usually mediate growth effects. When injected into mammary fat pads of ovariectomized nude mice implanted with estrogen pellets, MCF-7/SPHK1 cells formed more and larger tumors than vector transfectants with higher microvessel density in their periphery. Collectively, our results suggest that SPHK1 may play an important role in breast cancer progression by regulating tumor cell growth and survival.