Metabolic pathway visualization in living yeast by DNP-NMR

Central carbon metabolism of living Saccharomyces cerevisiae is visualized by DNP-NMR. Experiments are conducted as real time assays that detect metabolic bottlenecks, pathway use, reversibility of reactions and reaction mechanisms in vivo with subsecond time resolution.

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Bibliographic Details
Published inMolecular bioSystems Vol. 7; no. 10; pp. 2834 - 2836
Main Authors Meier, Sebastian, Karlsson, Magnus, Jensen, Pernille R, Lerche, Mathilde H, Duus, Jens Ø
Format Journal Article
LanguageEnglish
Published England 01.01.2011
Subjects
Carbon
Magnetic Resonance Spectroscopy - methods
Metabolic pathways
Reaction mechanisms
Saccharomyces cerevisiae
Saccharomyces cerevisiae - metabolism
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Summary:Central carbon metabolism of living Saccharomyces cerevisiae is visualized by DNP-NMR. Experiments are conducted as real time assays that detect metabolic bottlenecks, pathway use, reversibility of reactions and reaction mechanisms in vivo with subsecond time resolution.
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ISSN:1742-206X
1742-2051
DOI:10.1039/c1mb05202k