Selective suppression of oral allergen-induced anaphylaxis by Allergin-1 on basophils in mice
Abstract Mast cells (MCs) play a critical role in oral allergen-induced anaphylaxis. However, the contribution of basophils to the anaphylaxis remains unclear. The inhibitory immunoreceptor Allergin-1 is highly expressed on MCs and basophils and inhibits FcεRI-mediated signaling in MCs. Here, we sho...
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Published in | International immunology Vol. 32; no. 3; pp. 213 - 219 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
UK
Oxford University Press
07.03.2020
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Subjects | |
Online Access | Get full text |
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Summary: | Abstract
Mast cells (MCs) play a critical role in oral allergen-induced anaphylaxis. However, the contribution of basophils to the anaphylaxis remains unclear. The inhibitory immunoreceptor Allergin-1 is highly expressed on MCs and basophils and inhibits FcεRI-mediated signaling in MCs. Here, we show that Allergin-1-deficient (Milr1−/−) mice developed more severe hypothermia, a higher mortality rate and a greater incidence of diarrhea than did wild-type (WT) mice in an oral ovalbumin (OVA)-induced food allergy model. MC-deficient Mas–TRECK mice, which had been reconstituted with either WT or Milr1−/− bone marrow-derived cultured MCs, did not develop hypothermia in this food allergy model. On the other hand, depletion of basophils by injection of anti-CD200R3 antibody rescued Milr1−/− mice from lethal hypothermia but not from diarrhea. In vitro analyses demonstrated that Allergin-1 inhibits IgE-dependent activation of both human and mouse basophils. Thus, Allergin-1 on basophils selectively suppresses oral allergen-induced anaphylaxis.
Basophil Allergin-1 inhibits oral allergen-induced anaphylaxis |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1460-2377 1460-2377 |
DOI: | 10.1093/intimm/dxz075 |