Analysis of replication region of the cryptic plasmid pAG20 from Acetobacter aceti 3620
The DNA sequence of small cryptic plasmid pAG20 in Acetobacter aceti was determined at 3064 bp with 51.6% GC pairs. The plasmid encoded a 186 amino acid protein which is important for plasmid replication in Gram-negative bacteria except Escherichia coli. Two 21 bp large direct repeat sequence 1 and...
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Published in | Biochemical and biophysical research communications Vol. 328; no. 1; pp. 27 - 31 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
04.03.2005
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Subjects | |
Online Access | Get full text |
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Summary: | The DNA sequence of small cryptic plasmid pAG20 in
Acetobacter aceti was determined at 3064
bp with 51.6% GC pairs. The plasmid encoded a 186 amino acid protein which is important for plasmid replication in Gram-negative bacteria except
Escherichia coli. Two 21
bp large direct repeat sequence 1 and two 13
bp direct repeat sequence 2 were determined in the regulation region upstream from gene encoded Rep protein. Vector pAG24 with kanamycin gene and two deletion derivatives pAG25 and pAG26 without
rep gene from plasmid pAG20 were constructed. Plasmid pAG24 was replicated in a broad host range like
E. coli,
Acetobacter pasteurianus,
A. aceti,
Comanomonas spp.,
Serratia marcescens, and
Shigella spp. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0006-291X 1090-2104 |
DOI: | 10.1016/j.bbrc.2004.12.136 |