Analysis of replication region of the cryptic plasmid pAG20 from Acetobacter aceti 3620

• Published in: Biochemical and biophysical research communications Vol. 328; no. 1; pp. 27 - 31
• Main Authors: Kretová, Miroslava, Szemeš, Tomáš, Laco, Juraj, Gronesová, Paulína, Grones, Jozef
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 04.03.2005
• Subjects: Acetobacter - genetics; Acetobacter - metabolism; Acetobacter aceti; Acetobacter pasteurianus; Acetobactor aceti; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Base Sequence; DNA, Bacterial - genetics; Escherichia coli; Molecular Sequence Data; Plasmid; Plasmids - genetics; Replicon - genetics; Replicon region; Sequence Analysis, DNA - methods; Sequence Homology, Nucleic Acid; Serratia marcescens; Shigella; Plasmid; Acetobactor aceti; Replicon region
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2004.12.136

The DNA sequence of small cryptic plasmid pAG20 in Acetobacter aceti was determined at 3064 bp with 51.6% GC pairs. The plasmid encoded a 186 amino acid protein which is important for plasmid replication in Gram-negative bacteria except Escherichia coli. Two 21 bp large direct repeat sequence 1 and two 13 bp direct repeat sequence 2 were determined in the regulation region upstream from gene encoded Rep protein. Vector pAG24 with kanamycin gene and two deletion derivatives pAG25 and pAG26 without rep gene from plasmid pAG20 were constructed. Plasmid pAG24 was replicated in a broad host range like E. coli, Acetobacter pasteurianus, A. aceti, Comanomonas spp., Serratia marcescens, and Shigella spp.