Black shank resistant tobacco by silencing of glutathione S-transferase

A glutathione S-transferase gene was amplified from cDNA of Nicotiana tabacum roots infected with Phytophthora parasitica var. nicotianae. The gene was cloned in sense and anti-sense orientation to an RNAi vector for induced gene silencing, and reduced expression of the gene was detected by RT-PCR....

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Published inBiochemical and biophysical research communications Vol. 387; no. 2; pp. 300 - 304
Main Authors Hernández, Ingrid, Chacón, Osmany, Rodriguez, Raisa, Portieles, Roxana, López, Yunior, Pujol, Merardo, Borrás-Hidalgo, Orlando
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 18.09.2009
Subjects
Cloning, Molecular
Disease resistance
Gene Silencing
Glutathione S-transferase
Glutathione Transferase - genetics
Nicotiana - genetics
Nicotiana tabacum
Phytophthora
Phytophthora parasitica
Plant Diseases - genetics
Plant Diseases - parasitology
RNAi
Tobacco black shank
Disease resistance
RNAi
Tobacco black shank
Glutathione S-transferase
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Summary:A glutathione S-transferase gene was amplified from cDNA of Nicotiana tabacum roots infected with Phytophthora parasitica var. nicotianae. The gene was cloned in sense and anti-sense orientation to an RNAi vector for induced gene silencing, and reduced expression of the gene was detected by RT-PCR. A statistically significant increase in resistance of N. tabacum to infection following gene silencing was found for glutathione S-transferase-silenced plants compared with control plants. Some defense genes were up-regulated in glutathione S-transferase-silenced plants during the interaction with the pathogen. This is the first evidence of the role of glutathione S-transferase as negative regulator of defense response.
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ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2009.07.003