Retained efficacy of ivermectin against cyathostomins in Swedish horse establishments practicing selective anthelmintic treatment

• Published in: Veterinary parasitology Vol. 322; p. 110007
• Main Authors: Alm, Ylva Hedberg, Osterman-Lind, Eva, Martin, Frida, Lindfors, Rebecca, Roepstorff, Nina, Hedenström, Ulf, Fredriksson, Isabelle, Halvarsson, Peter, Tydén, Eva
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.10.2023
• Subjects: Anthelmintic resistance; Cyathostominae; Cyathostomins; ERP; FECRT; Ivermectin; Macrocyclic lactones; Small strongyles; Cyathostomins; ERP; Small strongyles; Macrocyclic lactones; Anthelmintic resistance; Cyathostominae; FECRT; Ivermectin
• ISSN: 0304-4017; 1873-2550
• DOI: 10.1016/j.vetpar.2023.110007

Cyathostominae are ubiquitous to grazing horses and regarded the most prevalent internal parasite in the horse. Unfortunately, decades of indiscriminate use of anthelmintic drugs have resulted in the development of resistance in cyathostomins to all currently available drug groups, the most recent being a documented lack of efficacy to the macrocyclic lactones (ML). In vivo determination of anthelmintic resistance in horses most often utilises the faecal egg count reduction test (FECRT). Further, a shortened egg reappearance period (ERP) can indicate a change in response to the applied treatment and suggest an upcoming reduction of efficacy. Although both true resistance as demonstrated by the FECRT and shorter ERPs after ML treatment have now been shown in cyathostomins worldwide, the efficacy of ML as regards to cyathostomins in Sweden is currently unknown. The aim of the present study was therefore to determine FECRTs and ERPs after ivermectin (IVM) treatment in Swedish horses. Sixteen equestrian establishments with a minimum of six horses excreting at least 150 eggs per gram faeces (EPG) at screening were selected. For each establishment, FECRTs and ERPs were determined by collecting faecal samples prior to and 14 days after IVM treatment (200 µg/kg), and thereafter at weekly intervals for a total of eight weeks. All participants responded to a questionnaire detailing pasture management methods and anthelmintic routines.Questionnaire results showed that the majority of establishments (69%) only treated horses with anthelmintic drugs if indicated by faecal diagnostics and all of the establishments had a mean FECRT exceeding 99.0% and ERPs ranging from six to over eight weeks. The ERP was shown to increase with age as young individuals were shown to excrete cyathostomin eggs earlier after treatment compared with older horses (R = 0.21, p = 0.015). Riding schools, stud farms and those declaring not to use separate summer and winter paddocks had significantly shorter ERPs (p <0.01).In conclusion, retained ERPs and no confirmed resistance to IVM were found in Swedish equine establishments practising selective anthelmintic treatment, and supports the use of selective deworming regimens as a means of reducing the risk of anthelmintic resistance development. [Display omitted] •Routine anthelmintic treatment of horses without prior diagnostics is rare in Sweden.•Ivermectin resulted in FECRs > 99% in all studied horse establishments.•ERPs after ivermectin treatment were 6–8 weeks in all studied horse establishments.•No evidence of resistance in cyathostomins to ivermectin shown in Swedish horses.•Selective treatment regimens can reduce anthelmintic resistance development.