Analysis of Antibody Response to HPV 16 and HPV 18 Antigens in Tunisian Patients

• Published in: Viral immunology Vol. 22; no. 1; pp. 7 - 16
• Main Authors: Achour, Mongia, Kahla, Saloua, Zeghal, Dorra, Kochbati, Lotfi, Mongi, Maalej, Zouari, Faouzia, Oueslati, Ridha
• Format: Journal Article
• Language: English
• Published: United States Mary Ann Liebert, Inc 01.02.2009
• Subjects: Adult; Aged; Aged, 80 and over; Antibodies, Viral - blood; Antibody Specificity; Capsid Proteins - genetics; Capsid Proteins - immunology; Escherichia coli; Female; Human papillomavirus; Human papillomavirus 16 - immunology; Human papillomavirus 18 - immunology; Humans; Humoral Immunity; Middle Aged; Oncogene Proteins, Viral - genetics; Oncogene Proteins, Viral - immunology; Papillomavirus E7 Proteins - genetics; Papillomavirus E7 Proteins - immunology; Papillomavirus Infections - epidemiology; Papillomavirus Infections - immunology; Papillomavirus Infections - virology; Recombinant Proteins - immunology; Repressor Proteins - genetics; Repressor Proteins - immunology; Tunisia - epidemiology; Uterine Cervical Neoplasms - epidemiology; Uterine Cervical Neoplasms - immunology; Uterine Cervical Neoplasms - virology
• ISSN: 0882-8245; 1557-8976
• DOI: 10.1089/vim.2008.0009

Objective: The aim of this present study was to use Luminex technology to detect antibodies against the late antigen L1 as well as those directed against the early antigens E6 and E7. Background Data: Human papillomavirus (HPV) serology is complex because infection and disease lead to distinct type-specific antibody responses. Materials and Methods: Viral antigens were expressed with pGEX vectors in Escherichia coli and then used in Luminex as coating antigens for antibody detection in 205 human sera samples: 71 cervical cancer cases, 64 cases of cervical inflammation, and 70 controls. Results: The data showed that 90.14% of sera among the cervical cancer patients had seropositivity toward at least one of the HPV 16 or HPV 18 antigens. Moreover, the percentages of positivity toward E6 and E7 HPV 16 antigens were 44% and 61%, respectively, versus only 21% for the L1 antigen. Among cervical cancer patients, the data showed different distributions in women of different ages. In addition, the intensity of the antibody response was also different for the six antigens analyzed. Conclusions: Antibody detection depends on the type of antigen, and is well correlated with international scientific findings. The differences in antibody response between patients with inflammation and patients with cervical cancer were significant.