Enhanced nerve growth factor efficiency in neural cell culture by immobilization on the culture substrate
Nerve growth factor (NGF) immobilization on a culture substrate may dramatically reduce the amount of NGF required for pheochromocytoma (PC12) cell culture. Coverslips on which NGF had been immobilized, or with NGF added to the culture medium daily, were used to culture PC12 cells. We examined the e...
Saved in:
Published in | Biochemical and biophysical research communications Vol. 382; no. 2; pp. 315 - 320 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
01.05.2009
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Nerve growth factor (NGF) immobilization on a culture substrate may dramatically reduce the amount of NGF required for pheochromocytoma (PC12) cell culture. Coverslips on which NGF had been immobilized, or with NGF added to the culture medium daily, were used to culture PC12 cells. We examined the effects of adding 5, 10, or 100ng of NGF to cultures daily, and compared them to the effects of immobilizing 5, 10, or 100ng of NGF on culture substrates in a single dose. Cultures with 10 or 5ng NGF added daily showed dramatically decreased cell viability, mitochondrial metabolic activity, and neuronal differentiation compared to cultures with 100ng NGF added daily, while also exhibiting increased apoptosis. In contrast, a single dose of 100ng immobilized NGF yielded results similar to 100ng NGF added daily (total: 300ng over 3days), and 10 or 5ng immobilized NGF showed far better results than 10 or 5ng NGF added daily. These results demonstrate that NGF immobilization can dramatically reduce the amount of NGF required in neuronal cell culture. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0006-291X 1090-2104 |
DOI: | 10.1016/j.bbrc.2009.03.016 |