Assignment of the TCRA/TCRD locus to sheep chromosome bands 7q1.4→q2.2 by fluorescence in situ hybridization

• Published in: Cytogenetic and genome research Vol. 79; no. 3-4; pp. 193 - 195
• Main Authors: MASSARI, S, ANTONACCI, R, DE CARO, F, LIPSI, M. R, CICCARESE, S
• Format: Journal Article
• Language: English
• Published: Freiburg Karger 01.01.1997; Basel S. Karger AG; Paris
• Subjects: Animals; Biological and medical sciences; Chromosome Mapping; Classical genetics, quantitative genetics, hybrids; Fundamental and applied biological sciences. Psychology; Genetics of eukaryotes. Biological and molecular evolution; In Situ Hybridization, Fluorescence; Molecular Sequence Data; Receptors, Antigen, T-Cell, alpha-beta - genetics; Receptors, Antigen, T-Cell, gamma-delta - genetics; Sheep - genetics; Vertebrata; Genetic mapping; Vertebrata; Mammalia; T cell receptor; Gene; Synteny; Chromosome 7; Sheep; Artiodactyla; Ungulata; V segment
• ISSN: 0301-0171; 1424-8581; 1424-859X
• DOI: 10.1159/000134718

Two genomic clones for the TCRA/TCRD locus have been isolated and characterized in sheep. The first clone corresponds to a new sheep Valpha element, the other to the entire Cdelta gene. Chromosomal mapping by fluorescence in situ hybridization (FISH) of Valpha and Cdelta clones localized the TCRA/TCRD locus on sheep chromosome region 7q14-->q22. This is the first physical assignment for genomic clones on sheep chromosome 7 by FISH. Moreover, the present data put the ovine 7q14-->q22 and the human 14q11.2 regions in the same syntenic group.