Berberine sensitizes ovarian cancer cells to cisplatin through miR-21/PDCD4 axis

• Published in: Acta biochimica et biophysica Sinica Vol. 45; no. 9; pp. 756 - 762
• Main Authors: Liu, Shiguo, Fang, Yue, Shen, Huiling, Xu, Wenlin, Li, Hao
• Format: Journal Article
• Language: English
• Published: China 01.09.2013
• Subjects: 3' Untranslated Regions - genetics; Antineoplastic Agents - pharmacology; Apoptosis - drug effects; Apoptosis - genetics; Apoptosis Regulatory Proteins - genetics; Apoptosis Regulatory Proteins - metabolism; Berberine - pharmacology; Blotting, Western; Cell Line, Tumor; Cell Survival - drug effects; Cell Survival - genetics; Cisplatin - pharmacology; Drug Resistance, Neoplasm - genetics; Drug Synergism; Female; Gene Expression Regulation, Neoplastic - drug effects; Humans; MicroRNAs - genetics; Mutation; Oligonucleotides, Antisense - genetics; Ovarian Neoplasms - genetics; Ovarian Neoplasms - metabolism; Ovarian Neoplasms - pathology; Reverse Transcriptase Polymerase Chain Reaction; RNA Interference; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; 卵巢癌细胞; 敏感性; 细胞凋亡; 细胞株; 联合培养; 轴; 顺铂; 黄连素; ovarian cancer; miR-21; berberine
• ISSN: 1672-9145; 1745-7270
• DOI: 10.1093/abbs/gmt075

Recent studies have shown that mieroRNA-21 （miR-21） con- tributes to tumor resistance to chemotherapy. Interestingly, we have found that berberine could inhibit miR-21 expres- sion in several cancer cell lines. In this study, we investigated whether berberine could modulate the sensitivity of ovarian cancer cells to cisplatin and explored the mechanism. The cisplatin-resistant SKOV3 cells that were incubated with berberine combined with cisplatin had a significantly lower survival than the cisplatin alone group and enhanced cis- platin-induced apoptosis. Berberine could inhibit miR-21 expression and function in ovarian cancer, as shown by an enhancement of its target PDCD4, an important tumor sup- pressor in ovarian cancer. The results suggested that berber- ine could modulate the sensitivity of cisplatin via regulating miR-21/PDCD4 axis in the ovarian cancer cells.