Collagen-chitosan scaffold modified with Au and Ag nanoparticles: Synthesis and structure

• Published in: Applied surface science Vol. 366; pp. 365 - 371
• Main Authors: Rubina, M.S., Kamitov, E.E., Zubavichus, Ya. V., Peters, G.S., Naumkin, A.V., Suzer, S., Vasil’kov, A.Yu
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15.03.2016
• Subjects: Agglomeration; Collagen-chitosan scaffolds; Gold; Metal-vapor synthesis; Nanocomposite materials; Nanoparticles; SAXS; Scaffolds; Silver; Synthesis; X-ray photoelectron spectroscopy; XANES/EXAFS; XPS; XRD; Nanoparticles; Metal-vapor synthesis; XANES/EXAFS; XPS; XRD; Collagen-chitosan scaffolds; SAXS
• ISSN: 0169-4332; 1873-5584
• DOI: 10.1016/j.apsusc.2016.01.107

•Biocompatible collagen-chitosan scaffolds were modified by Au and Ag nanoparticles via the metal-vapor synthesis.•Structural and morphological parameters of the nanocomposites were assessed using a set of modern instrumental techniques, including electron microscopy, X-ray diffraction, small-angle X-ray scattering, EXAFS, XPS.•Potential application of the nanocomposites are envisaged. Nowadays, the dermal biomimetic scaffolds are widely used in regenerative medicine. Collagen-chitosan scaffold one of these materials possesses antibacterial activity, good compatibility with living tissues and has been already used as a wound-healing material. In this article, collagen-chitosan scaffolds modified with Ag and Au nanoparticles have been synthesized using novel method - the metal-vapor synthesis. The nanocomposite materials are characterized by XPS, TEM, SEM and synchrotron radiation-based X-ray techniques. According to XRD data, the mean size of the nanoparticles (NPs) is 10.5nm and 20.2nm in Au-Collagen-Chitosan (Au-СollСh) and Ag-Сollagen-Сhitosan (Ag-CollCh) scaffolds, respectively in fair agreement with the TEM data. SAXS analysis of the composites reveals an asymmetric size distribution peaked at 10nm for Au-CollCh and 25nm for Ag-CollCh indicative of particle's aggregation. According to SEM data, the metal-carrying scaffolds have layered structure and the nanoparticles are rather uniformly distributed on the surface material. XPS data indicate that the metallic nanoparticles are in their unoxidized/neutral states and dominantly stabilized within the chitosan-rich domains.