CRISPR–Cas system and its use in the diagnosis of infectious diseases

• Published in: Microbiological research Vol. 263; p. 127100
• Main Authors: Padmanaban, Venkatesan, Ranganathan, Uma Devi K.
• Format: Journal Article
• Language: English
• Published: Elsevier GmbH 01.10.2022
• Subjects: CRISPR-Cas; Infectious disease; Molecular diagnosis; Point of care; CRISPR-Cas; Infectious disease; Molecular diagnosis; Point of care
• ISSN: 0944-5013; 1618-0623
• DOI: 10.1016/j.micres.2022.127100

Rapid and accurate diagnostic methods for detecting pathogens are needed for effective management and treatment of infectious diseases. The conventional pathogen detection approach based on culture is considered the gold standard method, but needs several days to corroborate its results. Using nucleic acids from pathogens as detection targets has a considerable advantage in overcoming these time-consuming issues. The development of several molecular techniques has started to change the landscape of infectious disease diagnosis. However, these require expensive reagents, equipment, and sophisticated infrastructure, as well as highly trained workers. In this context, it is necessary to identify new diagnostic strategies to overcome these issues. Recently, CRISPR/Cas based diagnosis has revolutionized the area of molecular diagnostics of pathogenic diseases. In this review, we have discussed the different classes of CRISPR-Cas systems and their functions, and then focused on recent advances in CRISPR-based diagnosis technologies and the perspective of using this as a potential biosensing platform to detect infectious disease.