Mdivi-1 Induced Mitochondrial Fusion as a Potential Mechanism to Enhance Stress Tolerance in Wheat

Mitochondria play a key role in providing energy to cells. These organelles are constantly undergoing dynamic processes of fusion and fission that change in stressful conditions. The role of mitochondrial fusion in wheat root cells was studied using Mdivi-1, an inhibitor of the mitochondrial fragmen...

Full description

Saved in:
Bibliographic Details
Published inLife (Basel, Switzerland) Vol. 12; no. 9; p. 1386
Main Authors Rakhmatullina, Daniya, Mazina, Anastasia, Ponomareva, Anastasia, Dmitrieva, Svetlana, Beckett, Richard Peter, Minibayeva, Farida
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 06.09.2022
MDPI
Subjects
Activation energy
Apoptosis
Autophagy
Cell cycle
Cell fusion
Energy balance
Energy metabolism
energy status
Fission
fusion
Genes
Glyceraldehyde-3-phosphate dehydrogenase
Glycolysis
Kinases
Lipid peroxidation
Mammals
Membrane fusion
Metabolism
Microscopy
Mitochondria
Morphology
Organelles
Phosphorylation
Physiology
Proteins
redox metabolism
Respiration
Roots
Seedlings
Seeds
Stress
Sucrose
Wheat
Wounding
Germany
Online AccessGet full text

Cover

More Information
Summary:Mitochondria play a key role in providing energy to cells. These organelles are constantly undergoing dynamic processes of fusion and fission that change in stressful conditions. The role of mitochondrial fusion in wheat root cells was studied using Mdivi-1, an inhibitor of the mitochondrial fragmentation protein Drp1. The effect of the inhibitor was studied on mitochondrial dynamics in the roots of wheat seedlings subjected to a wounding stress, simulated by excision. Treatment of the stressed roots with the inhibitor increased the size of the mitochondria, enhanced their functional activity, and elevated their membrane potentials. Mitochondrial fusion was accompanied by a decrease in ROS formation and associated cell damage. Exposure to Mdivi-1 also upregulated genes encoding the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and an energy sensor AMP-dependent protein sucrose non-fermenting-related kinase (SnRK1), suggesting that mitochondrial fusion is associated with a general activation of energy metabolism. Controlling mitochondrial fusion rates could change the physiology of wheat plants by altering the energy status of the cell and helping to mitigate the effects of stress.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2075-1729
2075-1729
DOI:10.3390/life12091386