Polyclonal antibodies against a structure mimicking the covalent linkage unit between picornavirus RNA and VPg: an immunochemical study

• Published in: Biochemistry (Moscow) Vol. 70; no. 9; pp. 1038 - 1045
• Main Authors: Ivanova, O A, Venyaminova, A G, Repkova, M N, Drygin, Yu F
• Format: Journal Article
• Language: English
• Published: United States Springer 01.09.2005; Springer Nature B.V
• Subjects: Animals; Antibodies; Antibodies - immunology; Antibodies - metabolism; Antibody Specificity; Bacteria; Chromatography; Crotalus - immunology; Crotalus - metabolism; Deoxyribonucleic acid; DNA; Encephalomyocarditis virus; Encephalomyocarditis virus - immunology; Encephalomyocarditis virus - metabolism; Haptens - immunology; Haptens - metabolism; Humans; Immunohistochemistry; Isomerases; Picornaviridae - enzymology; Picornaviridae - immunology; Picornavirus; Picornavirus infections; Poisons; Polyclonal antibodies; Proteins; RNA, Viral - immunology; RNA, Viral - metabolism; Tyrosine - immunology; Tyrosine - metabolism; Viral antibodies; Viral Proteins - immunology; Viral Proteins - metabolism
• ISSN: 0006-2979; 1608-3040; 0320-9725
• DOI: 10.1007/s10541-005-0222-0

We propose that therapy of patients with anticancer drugs that poison DNA topoisomerases induces formation of covalent complexes of cellular RNAs and DNA topoisomerases. The appearance of these complexes can be detected with antibodies against a synthetic hapten mimicking the covalent linkage unit Tyr-pU(p) of picornavirus RNA and VPg. We synthesized hapten [N(Ac),CO(NH2)]Tyr-(5 P --> O)Up-O-(CH2)6NH2, conjugated it with BSA, and immunized rabbits with the antigen obtained. The raised polyclonal antibodies were purified by successive affinity chromatography on BSA-Sepharose and hapten-Sepharose columns. Target antibodies recognized hapten and encephalomyocarditis virus RNA-VPg complex specifically as found using the dot-immunogold method. We believe that these antibodies might be useful to study mechanism of picorna and similar virus RNA synthesis. The discovery and qualitative determination of the cellular RNA-DNA topoisomerases covalent complexes with these antibodies might be useful to monitor therapy efficacy by drugs "freezing" dead-end complexes of DNA topoisomerases and nucleic acids and to understand the mechanism of DNA topoisomerase poisoning in situ.