Mapping of QTLs in tomato line FLA456 associated with resistance to a virus causing tomato yellow leaf curl disease

• Published in: Euphytica Vol. 190; no. 2; pp. 297 - 308
• Main Authors: Kadirvel, P., de la Peña, R., Schafleitner, R., Huang, S., Geethanjali, S., Kenyon, L., Tsai, W., Hanson, P.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.04.2013; Springer; Springer Nature B.V
• Subjects: Agronomy. Soil science and plant productions; Analysis; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Chromosomes; Classical and quantitative genetics. Population genetics. Molecular genetics; Fundamental and applied biological sciences. Psychology; Gene mapping; Generalities. Genetics. Plant material; Genes; Genetic translation; Genetics; Genetics and breeding of economic plants; Life Sciences; Molecular genetics; Pest resistance; Phenotypic variations; Plant diseases; Plant Genetics and Genomics; Plant pathogens; Plant Pathology; Plant Physiology; Plant Sciences; Quantitative genetics; Tomatoes; Tropical environments; Varietal selection. Specialized plant breeding, plant breeding aims; Vegetable industry; Geminivirus; QTL; SSR; Virus resistance; Begomovirus; Cartography; Tomato yellow leaf curl virus; Plant pathogen; Disease; Solanum lycopersicum; Tomato; Virus; Quantitative trait loci; Microorganism resistance; Vegetable crop; Dicotyledones; Microsatellite DNA; Angiospermae; Genetic improvement; Lycopersicon esculentum; Geminiviridae; Spermatophyta; Solanaceae
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1007/s10681-012-0848-0

Tomato (yellow) leaf curl disease (TYLCD) is a serious threat to tomato production in the tropics and subtropics. The genetics of resistance to Tomato yellow leaf curl Thailand virus Taiwan strain (TYLCTHV-[TW]) in a highly resistant tomato line FLA456 was studied through quantitative trait loci (QTL) analysis. Four QTLs named qTy4.1, qTy6.1, qTy10.1 and qTy11.1 were detected on chromosomes 4, 6, 10, and 11, respectively, through evaluation of an F 6 recombinant inbred line (RIL) population derived from a cross between FLA456 (resistant) and CLN1621L (susceptible). Gene action of all QTLs was recessive based on disease reaction of the F 1 . The markers SINAC1 and SLM4-34 flanked qTy4.1 on chromosome 4, and SLM11-12 and SLM11-17 defined qTy11.1, which co-located with the previously identified Ty - 5 and Ty - 2 loci, respectively. qTy6.1 was flanked by the markers SLM6-55 and TES-0014, and qTy10.1 by the markers SLM10-80-SLM10-46 on chromosomes 6 and 10. The LOD values of the putative QTLs ranged from 2.79 to 13.76. The phenotypic variance explained by each QTL ranged from 7.1 to 31.9 %. The four QTLs collectively contributed about 60.5 % of the phenotypic variation in resistance against TYLCTHV-[TW]. Group mean severity scores of those RILs possessing three or four qTy were generally lower than RIL groups with only one or no qTy. Given the diversity of begomoviruses that cause TYLCD across the regions, the new QTLs from FLA456 would be valuable in tomato breeding for developing varieties with durable resistance. Two QTL intervals (qTy4.1 and qTy10.1) contained virus resistance candidate genes such as CTV.22 and eukaryotic translation initiation factor 4E.