CRISPR/Cas9-targeted mutagenesis of a representative member of a novel PR10/Bet v1-like protein subfamily significantly reduces rice plant height and defense against Meloidogyne graminicola
Abstract Plant PR10/Bet v1-like proteins are involved in defense against microbial pathogens, however, whether they participate in host defense against plant-parasitic animals including nematodes remains unknown. Here, according to the phylogeny, sequence-similarity, and secondary structure analyses...
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Published in | Phytopathology research Vol. 4; no. 1; pp. 1 - 13 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
London
BioMed Central
17.10.2022
BMC |
Subjects | |
Online Access | Get full text |
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Summary: | Abstract
Plant PR10/Bet v1-like proteins are involved in defense against microbial pathogens, however, whether they participate in host defense against plant-parasitic animals including nematodes remains unknown. Here, according to the phylogeny, sequence-similarity, and secondary structure analyses, we found that the rice OsBet v1 protein and its homologs constitute a new subfamily of PR10/Bet v1-like protein. Reverse transcription-quantitative PCR assay showed that
OsBet v1
was highly expressed in rice roots and induced by
Meloidogyne graminicola
infection. Using a transient expression assay in rice protoplasts, we demonstrated that OsBet v1 was localized in the cytoplasm and nucleus. Furthermore, CRISPR/Cas9-targeted mutagenesis of
OsBet v1
significantly increased rice susceptibility to
M. graminicola
, but reduced rice plant height. In addition, the expression levels of two peroxidase genes (
peroxidase 5
and
peroxidase 56
) were significantly down-regulated in
OsBet v1
knockout mutants compared with those in wild-type rice plants. Taken together, this study identified a new PR10/Bet v1-like protein subfamily in plant and revealed the involvement of OsBet v1, a representative member of the subfamily, in rice growth and defense against
M. graminicola
. This provides a new insight into the role of plant PR10/Bet v1-like proteins in plant–nematode interactions. |
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ISSN: | 2524-4167 2096-5362 2524-4167 |
DOI: | 10.1186/s42483-022-00143-z |