The Regulation of Phosphorus Release by Penicillium chrysogenum in Different Phosphate via the TCA Cycle and Mycelial Morphology

• Published in: The journal of microbiology Vol. 61; no. 8; pp. 765 - 775
• Main Authors: Wang, Liyan, Tian, Da, Zhang, Xiaoru, Han, Mingxue, Cheng, Xiaohui, Ye, Xinxin, Zhang, Chaochun, Gao, Hongjian, Li, Zhen
• Format: Journal Article
• Language: English
• Published: Seoul The Microbiological Society of Korea 01.08.2023; Springer Nature B.V
• Subjects: Acids; Biomedical and Life Sciences; Calcium phosphates; Chitinase; citrate (si)-synthase; Citrate synthase; Citric acid; endo-1,4-beta-glucanase; ferric phosphate; Fungi; Gene expression; Genes; Iron phosphates; Life Sciences; Microbial Physiology and Biochemistry; Microbiology; Morphology; Mycelia; mycelium; Organic acids; Oxalic acid; Pellets; Penicillium chrysogenum; Phosphates; Phosphorus; Phosphorus cycle; pyruvate dehydrogenase (lipoamide); Pyruvic acid; Secretion; Tricalcium phosphate; Tricarboxylic acid cycle; Tricarboxylic acid cycle; Mycelial morphology; Insoluble phosphate dissolution; RNA-seq; Organic acids
• ISSN: 1225-8873; 1976-3794; 1976-3794
• DOI: 10.1007/s12275-023-00072-2

Phosphate-solubilizing fungi (PSF) efficiently dissolve insoluble phosphates through the production of organic acids. This study investigates the mechanisms of organic acid secretion by PSF, specifically Penicillium chrysogenum , under tricalcium phosphate (Ca 3 (PO 4 ) 2 , Ca–P) and ferric phosphate (FePO 4 , Fe–P) conditions. Penicillium chrysogenum exhibited higher phosphorus (P) release efficiency from Ca-P (693.6 mg/L) than from Fe–P (162.6 mg/L). However, Fe–P significantly enhanced oxalic acid (1193.7 mg/L) and citric acid (227.7 mg/L) production by Penicillium chrysogenum compared with Ca–P (905.7 and 3.5 mg/L, respectively). The presence of Fe–P upregulated the expression of genes and activity of enzymes related to the tricarboxylic acid cycle, including pyruvate dehydrogenase and citrate synthase. Additionally, Fe–P upregulated the expression of chitinase and endoglucanase genes, inducing a transformation of Penicillium chrysogenum mycelial morphology from pellet to filamentous. The filamentous morphology exhibited higher efficiency in oxalic acid secretion and P release from Fe–P and Ca–P. Compared with pellet morphology, filamentous morphology enhanced P release capacity by > 40% and > 18% in Ca–P and Fe–P, respectively. This study explored the strategies employed by PSF to improve the dissolution of different insoluble phosphates.