Bidirectional membrane molecule transfer between dendritic and T cells

• Published in: Biochemical and biophysical research communications Vol. 359; no. 2; pp. 202 - 208
• Main Authors: He, Tianpei, Tang, Chaoke, Liu, Yongqing, Ye, Zhenmin, Wu, Xiaochu, Wei, Yangdou, Moyana, Terence, Xiang, Jim
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 27.07.2007
• Subjects: B7-1 Antigen - biosynthesis; CD11c Antigen - biosynthesis; CD4-Positive T-Lymphocytes - metabolism; CD40 Antigens - biosynthesis; Cell Line; Confocal microscopy; Dendritic cells; Dendritic Cells - cytology; Dendritic Cells - metabolism; Endosomes - metabolism; Flow Cytometry; Green Fluorescent Proteins - metabolism; Humans; Lysosomes - metabolism; Membrane molecule transfer; Microscopy, Confocal; Ovalbumin - metabolism; Phenotype; T cells; T-Lymphocytes - metabolism; Flow cytometry; Confocal microscopy; Dendritic cells; T cells; Membrane molecule transfer
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2007.05.099

The acquisition of dendritic cell (DC) molecules by T cells has been previously reported. However, it remains unclear whether the transfer is only mono- or bidirectional. In this study, we incubated CMFDA-labeled ovalbumin (OVA)-pulsed DC2.4 (DC2.4 OVA) cells with Dil-labeled OT II CD4 + T cells and analyzed the potential bidirectional molecule transfer. We also assessed the distribution of internalized membrane using two engineered DC2.4/Ia bGFP and MF4/TCRCFP DC lines. Our findings showed that membrane molecule transfer is bidirectional. CD4 + T cells acquired Ia b, CD11c, CD40, and CD80 from DC2.4 OVA cells, and conversely DC2.4 OVA cells took up CD4, CD25, CD69, and T cell receptor from T cells. The internalized molecules acquired by T cells and DCs mostly localized in endosomes and lysosomes, respectively. Taken together, this study demonstrated a novel phenomenon of bidirectional membrane molecule transfer between DCs and T cells.