Simultaneous characterization and quantitation of 11 coumarins in Radix Angelicae Dahuricae by high performance liquid chromatography with electrospray tandem mass spectrometry

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 51; no. 3; pp. 599 - 605
• Main Authors: Zheng, Xuguang, Zhang, Xiaowei, Sheng, Xiaona, Yuan, Zhifang, Yang, Wei, Wang, Qiao, Zhang, Lantong
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 05.02.2010; Elsevier
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Characterization; Coumarin; Coumarins - analysis; Coumarins - chemistry; Drugs, Chinese Herbal - analysis; Drugs, Chinese Herbal - chemistry; Evaluation Studies as Topic; Fundamental and applied biological sciences. Psychology; General pharmacology; HPLC–ESI-MS/MS; Medical sciences; Pharmacology. Drug treatments; Plant Roots; Quantitation; Radix Angelicae Dahuricae; Spectrometry, Mass, Electrospray Ionization - methods; Spectrometry, Mass, Electrospray Ionization - standards; Tandem Mass Spectrometry - methods; Tandem Mass Spectrometry - standards; HPLC–ESI-MS/MS; Characterization; Radix Angelicae Dahuricae; Quantitation; Coumarin; Chemical analysis; Root; Pharmacognosy; Coumarine derivatives; HPLC chromatography; Lactone; Electrospray; Medicinal plant; Mass spectrometry MS/MS; Simultaneous measurement; Plant origin; HPLC―ESI-MS/MS; Quantitative analysis; Below ground plant part
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2009.09.030

A high performance liquid chromatography with electrospray tandem mass spectrometry (HPLC–ESI-MS/MS) method has been developed to characterize and quantify 11 coumarin compounds in Radix Angelicae Dahuricae simultaneously. By using this HPLC–ESI-MS/MS method, all 11 coumarins were separated and determined within 10 min. These coumarins were detected by ESI + ionization method and quantified by multiple reaction monitor (MRM). The linear regressions were acquired with r 2 > 0.995, respectively. The precision was evaluated by intra- and inter-day tests, and relative standard deviation (R.S.D.) values were reported within the range of 1.14–4.42% and 0.37–4.00%. The recovery studies for the quantified compounds were observed over the range of 92.1–105.6% with R.S.D. values less than 4.55%. It demonstrated that the method developed was successfully applied for identification and quantification of 11 coumarins in Radix Angelicae Dahuricae. The results showed that the contents of coumarins in Radix Angelicae Dahuricae were processed differently and varied significantly.