Beta-lactam biosynthesis in a gram-negative eubacterium: purification and characterization of isopenicillin N synthase from Flavobacterium sp. strain SC 12.154

• Published in: Journal of Bacteriology Vol. 171; no. 10; pp. 5720 - 5728
• Main Authors: PALISSA, H, VON DÖHREN, H, KLEINKAUF, H, HONG-HOI TING, BALDWIN, J. E
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.10.1989
• Subjects: Antibiotics (antibacterial agents, antifungal agents); Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents; Applied microbiology; beta-Lactamases - metabolism; Biological and medical sciences; Blotting, Western; Cephalosporins - metabolism; Chromatography, High Pressure Liquid; Disulfides - pharmacology; Enzymes - isolation & purification; Fermentation; Flavobacterium - enzymology; Flavobacterium - metabolism; Fundamental and applied biological sciences. Psychology; Hydrogen-Ion Concentration; Kinetics; Microbiology; Molecular Weight; Oxidoreductases; Sulfhydryl Compounds - pharmacology; Temperature; Time Factors; Temperature; Purification; Molecular weight determination; Enzyme; Lactam; Enzyme inhibitor; Environmental factor; Stimulation; Biosynthesis; Strain; Characterization; Antibiotic; Flavobacterium; Immunoblotting assay; PH; Bacteria; Kinetic parameter; Localization
• ISSN: 0021-9193; 1067-8832; 1098-5530
• DOI: 10.1128/jb.171.10.5720-5728.1989

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