Validated 1H and 13C Nuclear Magnetic Resonance Methods for the Quantitative Determination of Glycerol in Drug Injections

In the current study, we employed high-resolution proton and carbon nuclear magnetic resonance spectroscopy (1H and 13C NMR) for quantitative analysis of glycerol in drug injections without any complex pre-treatment or derivatization on samples. The established methods were validated with good speci...

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Bibliographic Details
Published inMolecules (Basel, Switzerland) Vol. 23; no. 5; p. 1177
Main Authors Lu, Jiaxi, Wang, Pengli, Wang, Qiuying, Wang, Yanan, Jiang, Miaomiao
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 15.05.2018
MDPI
Subjects
13C NMR
1H NMR
Calibration
Carbon 13
Glycerol
Integration
Linearity
Magnetic resonance spectroscopy
NMR
Nuclear magnetic resonance
Pretreatment
Quantitative analysis
Resonance
Sampling methods
Spectrum analysis
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Summary:In the current study, we employed high-resolution proton and carbon nuclear magnetic resonance spectroscopy (1H and 13C NMR) for quantitative analysis of glycerol in drug injections without any complex pre-treatment or derivatization on samples. The established methods were validated with good specificity, linearity, accuracy, precision, stability, and repeatability. Our results revealed that the contents of glycerol were convenient to calculate directly via the integration ratios of peak areas with an internal standard in 1H NMR spectra, while the integration of peak heights were proper for 13C NMR in combination with an external calibration of glycerol. The developed methods were both successfully applied in drug injections. Quantitative NMR methods showed an extensive prospect for glycerol determination in various liquid samples.
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content type line 23
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules23051177