Quantitative determination of surface concentration of human apolipoprotein H with capillary electrophoresis

The phospholipid monolayer at an air/water interface is widely used to mimic the biological membrane. The dynamic process of the protein or peptide interacting with lipid molecules can be reflected in the change in surface pressure of the monolayer. But the conventional method used to measure the su...

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Bibliographic Details
Published inIUBMB life Vol. 49; no. 5; p. 437
Main Authors Wang, S, Sun, Y, Ru, Q, Luo, G, Sui, S
Format Journal Article
LanguageEnglish
Published England 01.05.2000
Subjects
beta 2-Glycoprotein I
Cell Membrane - metabolism
Dimyristoylphosphatidylcholine - metabolism
Electrophoresis, Capillary - methods
Glycoproteins - biosynthesis
Humans
Indicators and Reagents - metabolism
Membranes, Artificial
Phosphatidylglycerols - metabolism
Phospholipids - metabolism
Time Factors
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Summary:The phospholipid monolayer at an air/water interface is widely used to mimic the biological membrane. The dynamic process of the protein or peptide interacting with lipid molecules can be reflected in the change in surface pressure of the monolayer. But the conventional method used to measure the surface pressure change gives results that cannot easily be correlated with the contribution of a single protein molecule. Previously, measuring the surface concentration of the protein molecules at the air/water interface has required the protein to be labeled with radioactivity or fluorescence. Here, a new method using capillary electrophoresis is introduced to measure the surface concentration of the protein. The results show at least two advantages of the new method: The numerical results of protein concentration can be obtained in a more precise and rapid way; and there is no need to label the protein sample or to build a special monolayer setup.
ISSN:1521-6543
DOI:10.1080/152165400410290