Prostaglandin E2 Dilates Intracerebral Arterioles When Applied to Capillaries: Implications for Small Vessel Diseases
Prostaglandin E 2 (PGE 2 ) has been widely proposed to mediate neurovascular coupling by dilating brain parenchymal arterioles through activation of prostanoid EP4 receptors. However, our previous report that direct application of PGE 2 induces an EP1-mediated constriction strongly argues against it...
Saved in:
Published in | Frontiers in aging neuroscience Vol. 13; p. 695965 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Lausanne
Frontiers Research Foundation
13.08.2021
Frontiers Media S.A |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Prostaglandin E
2
(PGE
2
) has been widely proposed to mediate neurovascular coupling by dilating brain parenchymal arterioles through activation of prostanoid EP4 receptors. However, our previous report that direct application of PGE
2
induces an EP1-mediated constriction strongly argues against its direct action on arterioles during neurovascular coupling, the mechanisms sustaining functional hyperemia. Recent advances have highlighted the role of capillaries in sensing neuronal activity and propagating vasodilatory signals to the upstream penetrating parenchymal arteriole. Here, we examined the effect of capillary stimulation with PGE
2
on upstream arteriolar diameter using an
ex vivo
capillary-parenchymal arteriole preparation and
in vivo
cerebral blood flow measurements with two-photon laser-scanning microscopy. We found that PGE
2
caused upstream arteriolar dilation when applied onto capillaries with an EC
50
of 70 nM. The response was inhibited by EP1 receptor antagonist and was greatly reduced, but not abolished, by blocking the strong inward-rectifier K
+
channel. We further observed a blunted dilatory response to capillary stimulation with PGE
2
in a genetic mouse model of cerebral small vessel disease with impaired functional hyperemia. This evidence casts previous findings in a different light, indicating that capillaries are the locus of PGE
2
action to induce upstream arteriolar dilation in the control of brain blood flow, thereby providing a paradigm-shifting view that nonetheless remains coherent with the broad contours of a substantial body of existing literature. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Cam Ha Tran, University of Nevada, Reno, United States; George C. Wellman, University of Vermont, United States; Eric Newman, University of Minnesota, United States Edited by: Anusha Mishra, Oregon Health and Science University, United States |
ISSN: | 1663-4365 1663-4365 |
DOI: | 10.3389/fnagi.2021.695965 |