Enantioseparations in capillary liquid chromatography and capillary electrochromatography using amylose tris(3,5-dimethylphenylcarbamate) in combination with aqueous organic mobile phase

• Published in: Journal of separation science Vol. 25; no. 10-11; pp. 653 - 660
• Main Authors: Chankvetadze, Lali, Kartozia, Irma, Yamamoto, Chiyo, Chankvetadze, Bezhan, Blaschke, Gottfried, Okamoto, Yoshio
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.07.2002; WILEY‐VCH Verlag; Wiley
• Subjects: 5-Dimethylphenylcarbamate; Amylose tris; Amylose tris(3,5‐Dimethylphenylcarbamate); Analysis; Analytical chemistry; Biological and medical sciences; Capillary electrochromatography; Capillary liquid chromatography; Chemistry; Chiral selector; Chromatographic methods and physical methods associated with chromatography; Enantioseparations; Exact sciences and technology; General pharmacology; Medical sciences; Other chromatographic methods; Pharmacology. Drug treatments; Capillary column; Separation; Amylose derivative; Etozolin; Nifurtimox; HPLC chromatography; Chiral stationary phase; Experimental study; Organic carbamate; Ambucetamide; Omeprazole; Norgestrel; Aminoglutethimide; Ultraviolet detector; Optical resolution; Enantiomer; Electrochromatography
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/1615-9314(20020701)25:10/11<653::AID-JSSC653>3.0.CO;2-T

In this study the potential of amylose tris(3,5‐Dimethylphenylcarbamate) (ADMPC) was evaluated for enantioseparations in capillary liquid chromatography (CLC) and capillary electrochromatography (CEC). This material was previously used for enantioseparations in CEC with nonaqueous alcohols and the first study in aqueous‐organic mobile phase indicates its potential for CEC enantioseparations. The effect of various experimental parameters on the separation characteristics was investigated in both CLC and CEC mode. Under optimized conditions the plate numbers in CEC mode reached 500 000 plates/m for thiourea and exceeded 240 000 plates/m for the enantiomers of some chiral compounds investigated. Comparative enantioseparations performed in CLC and CEC mode revealed significant advantages of CEC.