Active Flavonoids from Colubrina greggii var. greggii S. Watson against Clinical Isolates of Candida spp

Candida albicans is the most commonly implicated agent in invasive human fungal infections. The disease could be presented as minimal symptomatic candidemia or can be fulminant sepsis. Candidemia is associated with a high rate of mortality and high healthcare and hospitalization costs. The surveilla...

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Published inMolecules (Basel, Switzerland) Vol. 26; no. 19; p. 5760
Main Authors Melchor-Martínez, Elda M., Tamez-Fernández, Juan F., González-González, Gloria María, Silva-Mares, David A., Waksman-Minsky, Noemí, Pérez-López, Luis Alejandro, Rivas-Galindo, Verónica M.
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 23.09.2021
MDPI
Subjects
Acetic acid
Antifungal activity
Antifungal agents
bioassay-guided fractionation
Bioassays
Biological activity
Butanol
Candida
candida spp
Candidemia
Chromatography
Clinical isolates
Colubrina greggii
colubrina greggii var. greggii
Communication
Drugs
Ethyl acetate
Flavonoids
Flowers
Fluconazole
Fractionation
Fungal infections
Fungicides
Geographical distribution
Mycosis
Plant extracts
Sepsis
Mexico
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Summary:Candida albicans is the most commonly implicated agent in invasive human fungal infections. The disease could be presented as minimal symptomatic candidemia or can be fulminant sepsis. Candidemia is associated with a high rate of mortality and high healthcare and hospitalization costs. The surveillance programs have reported the distribution of other Candida species reflecting the trends and antifungal susceptibilities. Previous studies have demonstrated that C. glabrata more frequently presents fluconazole-resistant strains. Extracts from Mexican plants have been reported with activity against pulmonary mycosis, among them Colubrina greggii. In the present study, extracts from the aerial parts (leaves, flowers, and fruits) of this plant were evaluated against clinical isolates of several species of Candida (C. albicans, C. glabrata, C. parapsilosis, C. krusei, and C. tropicalis) by the broth microdilution assay. Through bioassay-guided fractionation, three antifungal glycosylated flavonoids were isolated and characterized. The isolated compounds showed antifungal activity only against C. glabrata resistant to fluconazole, and were non-toxic toward brine shrimp lethality bioassay and in vitro Vero cell line assay. The ethyl acetate and butanol extracts, as well as the fractions containing the mixture of flavonoids, were more active against Candida spp.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules26195760