Mouse Retinal Progenitor Cell Dynamics on Electrospun Poly (ϵ-Caprolactone)

Age-related macular degeneration, retinitis pigmentosa and glaucoma are among the many retinal degenerative diseases where retinal cell death leads to irreversible vision loss and blindness. Working toward a cell-replacement-based therapy for such diseases, a number of research groups have recently...

Full description

Saved in:
Bibliographic Details
Published inJournal of biomaterials science. Polymer ed. Vol. 23; no. 11; pp. 1451 - 1465
Main Authors Cai, Sophie, Smith, Meghan Elisabeth, Redenti, Stephen Michael, Wnek, Gary Edmund, Young, Michael Joseph
Format Journal Article
LanguageEnglish
Published England Routledge 01.01.2012
Subjects
Animals
biocompatibility
Caproates - chemistry
Cell Adhesion
Cell Culture Techniques
Cell Differentiation
Cell Movement
electrospinning
Lactones - chemistry
Materials Testing
Mice, Inbred C57BL
Mice, Knockout
polycaprolactone
Porosity
Progenitor cell
retina
Retina - physiology
Retina - surgery
Retina - transplantation
Rhodopsin - genetics
Rhodopsin - metabolism
scaffold
Stem Cell Transplantation - instrumentation
Stem Cell Transplantation - methods
Stem Cells - physiology
Tissue Culture Techniques
Tissue Scaffolds - chemistry
biocompatibility
electrospinning
retina
scaffold
polycaprolactone
Progenitor cell
Online AccessGet full text

Cover

More Information
Summary:Age-related macular degeneration, retinitis pigmentosa and glaucoma are among the many retinal degenerative diseases where retinal cell death leads to irreversible vision loss and blindness. Working toward a cell-replacement-based therapy for such diseases, a number of research groups have recently evaluated the feasibility of using retinal progenitor cells (RPCs) cultured and transplanted on biodegradable polymer substrates to replace damaged retinal tissue. Appropriate polymer substrate design is essential to providing a three-dimensional environment that can facilitate cell adhesion, proliferation and post-transplantation migration into the host environment. In this study, we have designed and fabricated a novel, ultra-thin electrospun poly(ϵ-caprolactone) (PCL) scaffold with microscale fiber diameters, appropriate porosity for infiltration by RPCs, and biologically compatible mechanical characteristics. We have verified that our electrospun PCL scaffold supports robust mouse RPC proliferation, adhesion, and differentiation in vitro, as well as migration into mouse retinal explants. These promising results make PCL a strong candidate for further development as a cell transplantation substrate in retinal regenerative research.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0920-5063
1568-5624
DOI:10.1163/092050611X584388