Ultrastructural feature of spermatogenic cells and spermatozoon in cultured burbot Lota lota

• Published in: Tissue & cell Vol. 61; pp. 1 - 7
• Main Authors: Dadras, Hadiseh, Golpour, Amin, Dzyuba, Borys, Kristan, Jiri, Policar, Tomas
• Format: Journal Article
• Language: English
• Published: Scotland Elsevier Ltd 01.12.2019; Elsevier Science Ltd
• Subjects: Animals; Cell differentiation; Cells, Cultured; Centrioles; Chromatin; Cytoplasm; Differentiation (biology); Electron microscopy; Fishes - metabolism; Flagella; Freshwater fish; Gametocytes; Germ cells; Lota lota; Male; Microtubules; Sertoli Cells - ultrastructure; Sperm; Spermatids; Spermatids - ultrastructure; Spermatocytes; Spermatogenesis; Spermatogenic cells; Spermatogonia; Spermatogonia - ultrastructure; Spermatozoa; Spermatozoa - ultrastructure; Taxonomy; Testis - cytology; Testis - ultrastructure; Transmission electron microscopy; Tubules; Ultrastructure; Testis ultrastructure; Electron microscopy; Spermatogenesis; Spermatogenic cells
• ISSN: 0040-8166; 1532-3072
• DOI: 10.1016/j.tice.2019.08.005

•Different stages of spermatogenesis process in Burbot, Lota lota was described.•Three distinct stages of cell differentiation can be identified during spermiogenesis.•Spermiogenesis stages are characterised by three distinct morphological changes. Testis development and ultrastructure of spermatogenic cells and spermatozoa of burbot Lota lota, a commercially important cold freshwater fish, were studied by light and transmission electron microscopy. Spermatogonia, spermatocytes, spermatids, and spermatozoa are distributed along the seminiferous tubules. Electron-dense bodies appear in germ cells from primary spermatogonia to secondary spermatocytes. We identified three distinct stages of spermatid cell differentiation based on chromatin condensation, development of the flagellum, formation of a nuclear fossa, and elimination of excess cytoplasm. Spermatozoa were anacrosomal and characterized by location of the centrioles outside the nuclear fossa and incomplete perpendicular arrangement of the centrioles. The sperm flagellum displayed an axoneme with nine doublets of peripheral microtubules and two central microtubules. These results provide valuable information for burbot taxonomy and may clarify the process of spermatogenesis for this species.