Histopathological Parameters of Canine Bone Marrow in Cell-Block Preparations

Studies using the cell-block technique for bone marrow (BM) analysis are limited in the veterinary literature. This work assessed whether the histopathological analysis of canine BM was feasible using cell-block cytoinclusions prepared from fine-needle sternal aspirate samples. Eight clinically heal...

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Published inTopics in companion animal medicine Vol. 45; p. 100552
Main Authors Varjão, Natasha Milen, Araújo, Iguaracyra Barreto de Oliveira, Hlavac, Nicole, Nunes, Talyta Lins, Varjão, Bruno Milen, de Pinho, Flaviane Alves, Barrouin-Melo, Stella Maria
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.11.2021
Elsevier Limited
Subjects
Animals
Automation
Biopsy
Biopsy, Fine-Needle - veterinary
Blood
Body fat
Bone Marrow
Computer architecture
cytoinclusion
dog
Dogs
hematology
iliac crest
Morphology
Parameters
Statistical analysis
Statistical methods
sternum
Veterinary medicine
cytoinclusion
hematology
sternum
dog
iliac crest
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Summary:Studies using the cell-block technique for bone marrow (BM) analysis are limited in the veterinary literature. This work assessed whether the histopathological analysis of canine BM was feasible using cell-block cytoinclusions prepared from fine-needle sternal aspirate samples. Eight clinically healthy young-to-middle-aged dogs underwent both fine-needle sternal aspiration for BM cell-block (BM-Cb) processing and iliac-crest BM core biopsy (BM-B). Histopathologic parameters were compared between the 2 methods. There were no statistically significant histopathological differences between hematopoietic tissue areas (P =  .6294) in the BM-Cb and BM-B sections, and they had similar microscopic characteristics and microarchitecture. Cellularity and reticulin-fiber staining were equivalent in the BM-Cb and BM-B preparations in 87.5% (7/8) and 100% (8/8) of the sections, respectively. However, the quantitative results of the megakaryocytic series differed between BM-Cb and BM-B in 37.5% (3/8) of the sections, and the myeloid:erythroid (M:E) ratios differed between the 2 methods in 25% (2/8). These preliminary data indicate that cell-block preparations made from sternal fine-needle aspiration samples warrant continued evaluation in a larger number of animals, including those with various diseases affecting the bone marrow.
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ISSN:1938-9736
1946-9837
1876-7613
DOI:10.1016/j.tcam.2021.100552