Antioxidant effect of vitamin E and 5-aminosalicylic acid on acrylamide induced kidney injury in rats

• Published in: Saudi medical journal Vol. 38; no. 2; pp. 132 - 137
• Main Authors: Rajeh, Nisreen A, Al-Dhaheri, Najlaa M
• Format: Journal Article
• Language: English
• Published: Saudi Arabia Prince Sultan Military Medical City (PSMMC) 01.02.2017; Saudi Medical Journal
• Subjects: Acrylamide - toxicity; Acute Kidney Injury - chemically induced; Acute Kidney Injury - pathology; Acute Kidney Injury - prevention & control; Animals; Antioxidants; Antioxidants - therapeutic use; Cytochrome; Dehydrogenases; Enzymes; Kidney - drug effects; Kidney - pathology; L-Lactate Dehydrogenase - metabolism; Laboratories; Male; Medical research; Mesalamine - therapeutic use; Mortality; Original; Oxidative stress; Rats; Rats, Wistar; Statistical analysis; Toxicity; Variance analysis; Vitamin E - therapeutic use; Germany
• ISSN: 0379-5284; 1658-3175
• DOI: 10.15537/smj.2017.2.16049

To explore renal toxicity caused by sub-acute exposure of acrylamide and to study the protective effect of 5-Aminosalicylic acid (5-ASA) and Vitamin E (vit-E)on Acrylamide (ACR) induced renal toxicity. Methods: This study was conducted at King Fahad Medical Research Centre, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia, between August and November 2015. A total of 49 adult Wistar rats (250 ± 20g) aged 60 days were kept in a controlled environment and used in the present study. The rats were divided into 7 groups (control, ACR alone, ACR+5-ASA, ACR+vit-E, ACR+ASA+vit-E, vit-E alone, and ASA alone). After 5 days of ACR oral gavage treatment, the rats were observed for 24 hours then killed. Histopathology for the kidney and lactate dehydrogenase assay were carried out.  Results: Acrylamide produced significant pathological changes in the kidney with acute tubular necrosis in the distal tubules that could be reversed by concomitant injection of rat with 5-ASA. Together with vitamin E, 5-ASA, showed maximum renal protection. No statistically significant difference was observed in either body weights or lactate dehydrogenase activity of ACR treated rats.  Conclusion: Acrylamide exposure leads to adverse clinical pathologies of renal tubules, which were reversed by a concomitant treatment with 5-ASA and vitamin-E.