Colorimetric assay for the quantification of serum iodixanol concentration and its application on estimation of glomerular filtration rate in cattle

A colorimetric assay used to quantify the non-ionic contrast medium iodixanol in sera was validated and compared with high-performance liquid chromatography. The application of this assay to estimate glomerular filtration rates (GFR) in cattle was examined. Serum iodixanol was de-iodinated by alkali...

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Published inResearch in veterinary science Vol. 93; no. 1; pp. 378 - 380
Main Authors Miura, Moe, Yamagishi, Norio, Sasaki, Kouya, Kim, Danil, Devkota, Bhuminand, Furuhama, Kazuhisa
Format Journal Article
LanguageEnglish
Published England Elsevier India Pvt Ltd 01.08.2012
Elsevier Limited
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Summary:A colorimetric assay used to quantify the non-ionic contrast medium iodixanol in sera was validated and compared with high-performance liquid chromatography. The application of this assay to estimate glomerular filtration rates (GFR) in cattle was examined. Serum iodixanol was de-iodinated by alkaline hydrolysis and the amount of released iodine was subsequently determined using a ceric arsenite method. There was a close correlation between the two methods using identical specimens. In clinically healthy cattle with different body weights, the reference value (166.3–178.8mL/min/m2) based on body surface area was fairly stable as compared with that (2.13–3.63mL/min/kg) based on body weight. Based on GFR data in healthy and renal-impaired cattle, when the GFR decreased to more than 60% of the reference value, serum urea nitrogen and creatinine concentrations increased. The colorimetric assay is a simple method for the estimation of GFR in cattle and requires no expensive equipment.
Bibliography:ObjectType-Article-1
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ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2011.07.010