Colorimetric assay for the quantification of serum iodixanol concentration and its application on estimation of glomerular filtration rate in cattle

• Published in: Research in veterinary science Vol. 93; no. 1; pp. 378 - 380
• Main Authors: Miura, Moe, Yamagishi, Norio, Sasaki, Kouya, Kim, Danil, Devkota, Bhuminand, Furuhama, Kazuhisa
• Format: Journal Article
• Language: English
• Published: England Elsevier India Pvt Ltd 01.08.2012; Elsevier Limited
• Subjects: Animals; Cattle; Cattle - blood; Cattle - physiology; Chromatography, High Pressure Liquid - veterinary; Colorimetric assay; Colorimetry - methods; Colorimetry - veterinary; Contrast Media - analysis; Female; Glomerular filtration rate (GFR); Glomerular Filtration Rate - physiology; Iodixanol; Laboratory animals; Male; Medical imaging; Personal relationships; Serum; Studies; Triiodobenzoic Acids - blood; Veterinary medicine; Japan; Colorimetric assay; Serum; Glomerular filtration rate (GFR); Cattle; Iodixanol
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2011.07.010

A colorimetric assay used to quantify the non-ionic contrast medium iodixanol in sera was validated and compared with high-performance liquid chromatography. The application of this assay to estimate glomerular filtration rates (GFR) in cattle was examined. Serum iodixanol was de-iodinated by alkaline hydrolysis and the amount of released iodine was subsequently determined using a ceric arsenite method. There was a close correlation between the two methods using identical specimens. In clinically healthy cattle with different body weights, the reference value (166.3–178.8mL/min/m2) based on body surface area was fairly stable as compared with that (2.13–3.63mL/min/kg) based on body weight. Based on GFR data in healthy and renal-impaired cattle, when the GFR decreased to more than 60% of the reference value, serum urea nitrogen and creatinine concentrations increased. The colorimetric assay is a simple method for the estimation of GFR in cattle and requires no expensive equipment.