Flow cytometric S-phase fraction contributes to diagnosis of diploid malignant salivary gland tumours

• Published in: International journal of oral and maxillofacial surgery Vol. 35; no. 10; pp. 947 - 950
• Main Authors: Driemel, O., Kraft, K., Hemmer, J.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 01.10.2006; Elsevier
• Subjects: Adenoma, Pleomorphic - diagnosis; Adenoma, Pleomorphic - genetics; Adenoma, Pleomorphic - pathology; Adult; Aged; Aged, 80 and over; Aneuploidy; Biological and medical sciences; Chromosome aberrations; Dentistry; Diagnosis, Differential; Diploidy; DNA flow cytometry; DNA, Neoplasm - analysis; Epidemiologic Methods; Female; Flow Cytometry - methods; Humans; Male; Medical genetics; Medical sciences; Middle Aged; Otorhinolaryngology. Stomatology; S Phase - genetics; S-phase fraction; Salivary Gland Neoplasms - diagnosis; Salivary Gland Neoplasms - genetics; Salivary Gland Neoplasms - pathology; salivary gland tumours; Salivary Glands - pathology; aneuploidy; S-phase fraction; DNA flow cytometry; salivary gland tumours; Chromosomal aberration; Flow cytometry; Stomatology; Aneuploidy; Salivary gland; Malignant tumor; Treatment; Surgery; DNA; S Phase; Diagnosis
• ISSN: 0901-5027; 1399-0020
• DOI: 10.1016/j.ijom.2006.03.025

DNA ploidy studies on salivary gland tumours have shown that the proportion of aneuploid cases, although confined to the malignant entities, is considerably lower than for other solid malignancies. To analyse whether the S-phase fraction (SPF) may contribute to discrimination of diploid malignant from benign tumours, DNA flow cytometric data from 45 different malignant salivary gland tumours was compared with that of 121 pleomorphic adenomas. All benign tumours were diploid. Twelve malignant tumours contained aneuploid cell populations. The SPF values for diploid malignancies ranged between 0.9% and 11.0% (mean 3.9%), and between 0.5% and 7.9% (mean 2.7%) for pleomorphic adenomas. A 4% cut-off value gained statistical significance for discriminating diploid malignant tumours from pleomorphic adenomas ( P < 0.01). The sensitivity for SPF > 4% was 46% and the positive predictive value was 40%. A sensitivity of 60% and a positive predictive value of 54% was achieved by combining aneuploidy and SPF > 4%. These results show that DNA flow cytometry may contribute to diagnostic assessment in salivary gland tumours.