Salmonella enterica Serovar Typhimurium Uses PbgA/YejM To Regulate Lipopolysaccharide Assembly during Bacteremia

• Published in: Infection and immunity Vol. 88; no. 1
• Main Authors: Cian, Melina B, Giordano, Nicole P, Masilamani, Revathi, Minor, Keaton E, Dalebroux, Zachary D
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 17.12.2019
• Subjects: Animals; Bacteremia - microbiology; Bacterial Infections; Genetic Complementation Test; Lipopolysaccharides - metabolism; Membrane Proteins - genetics; Membrane Proteins - metabolism; Mice; Salmonella Infections, Animal - microbiology; Salmonella typhimurium - metabolism; Salmonella typhimurium - pathogenicity; Sequence Deletion; Spotlight; Survival Analysis; Virulence; outer membrane; Salmonella; FtsH; capsule; fatty acid; LpxC; phospholipids; unsaturated; colonic acid; capsular polysaccharide; O antigen; nontyphoidal; glycerophospholipids; lipooligosaccharide; lipid A-core; two-component system; RcsF; cyclopropane; fatty acids; lipid A; Toll-like receptor 4; macrophage; LapB/YciM; saturated; cardiolipin; phosphatidylethanolamine; intracellular pathogen; facultatively intracellular pathogens; rifampin; LPS precursor; persistence; O polysaccharide; PhoP; lipopolysaccharide; core oligosaccharide; lipidomics; phosphatidylglycerol; PhoQ; endotoxin
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/IAI.00758-19

serovar Typhimurium ( Typhimurium) relies upon the inner membrane protein PbgA to enhance outer membrane (OM) integrity and promote virulence in mice. The PbgA transmembrane domain (residues 1 to 190) is essential for viability, while the periplasmic domain (residues 191 to 586) is dispensable. Residues within the basic region (residues 191 to 245) bind acidic phosphates on polar phospholipids, like for cardiolipins, and are necessary for salmonella OM integrity. Typhimurium bacteria increase their OM cardiolipin concentrations during activation of the PhoPQ regulators. The mechanism involves PbgA's periplasmic globular region (residues 245 to 586), but the biological role of increasing cardiolipins on the surface is not understood. Nonsynonymous polymorphisms in three essential lipopolysaccharide (LPS) synthesis regulators, (also known as ), , and , variably suppressed the defects in OM integrity, rifampin resistance, survival in macrophages, and systemic colonization of mice in the Δ mutant (in which the PbgA periplasmic domain from residues 191 to 586 is deleted). Compared to the OMs of the wild-type salmonellae, the OMs of the mutants had increased levels of lipid A-core molecules, cardiolipins, and phosphatidylethanolamines and decreased levels of specific phospholipids with cyclopropanated fatty acids. Complementation and substitution mutations in LapB and LpxC generally restored the phospholipid and LPS assembly defects for the mutants. During bacteremia, mice infected with the mutants survived and cleared the bacteria, while animals infected with wild-type salmonellae succumbed within 1 week. Remarkably, wild-type mice survived asymptomatically with salmonellae in their livers and spleens for months, but Toll-like receptor 4-deficient animals succumbed to these infections within roughly 1 week. In summary, Typhimurium uses PbgA to influence LPS assembly during stress in order to survive, adapt, and proliferate within the host environment.