Effects of feeding pasteurized waste milk to dairy calves on phenotypes and genotypes of antimicrobial resistance in fecal Escherichia coli isolates before and after weaning

• Published in: Journal of dairy science Vol. 100; no. 10; pp. 7967 - 7979
• Main Authors: Maynou, G., Migura-Garcia, L., Chester-Jones, H., Ziegler, D., Bach, A., Terré, M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2017
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; antimicrobial resistance; calf; Cattle; Drug Resistance, Multiple, Bacterial - genetics; Escherichia coli - drug effects; Escherichia coli - genetics; Feces - microbiology; Female; Genotype; Milk; Pasteurization; pasteurized waste milk; Phenotype; Weaning; antimicrobial resistance; pasteurized waste milk; calf
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.2017-13040

The aim of this study was to evaluate the effects of feeding pasteurized waste milk (pWM) to calves on antimicrobial resistance of fecal Escherichia coli at both phenotypic and genotypic levels. Fifty-two Holstein female calves (3 ± 1.3 d of age) were fed 1 of the 2 different types of milk: milk replacer (MR) without antimicrobials or pWM with β-lactam residues until weaning at 49 d of age. Fecal swabs of all calves were obtained on d 0, 35, and 56 of the study and 3 E. coli isolates per sample were studied. Phenotypic resistance was tested by the disk diffusion method against a panel of 12 antimicrobials. A total of 13 resistance genes consisting of β-lactam, sulfonamide, tetracycline, and aminoglycoside families were examined by PCR. Feeding pWM to calves increased the presence of phenotypic resistance to ampicillin, cephalotin, ceftiofur, and florfenicol in fecal E. coli compared with MR-fed calves. However, the presence of resistance to sulfonamides, tetracyclines, and aminoglycosides was common in dairy calves independent of their milk-feeding source, suggesting other factors apart from the feeding source are involved in the emergence of antimicrobial resistance.