Relative Roles of Listeriolysin O, InlA, and InlB in Listeria monocytogenes Uptake by Host Cells

• Published in: Infection and immunity Vol. 86; no. 10
• Main Authors: Phelps, Christopher C, Vadia, Stephen, Arnett, Eusondia, Tan, Yubo, Zhang, Xiaoli, Pathak-Sharma, Sarika, Gavrilin, Mikhail A, Seveau, Stephanie
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.10.2018
• Subjects: Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacterial Toxins - genetics; Bacterial Toxins - metabolism; Cadherins - genetics; Cadherins - metabolism; Cellular Microbiology: Pathogen-Host Cell Molecular Interactions; Heat-Shock Proteins - genetics; Heat-Shock Proteins - metabolism; Hemolysin Proteins - genetics; Hemolysin Proteins - metabolism; Hepatocytes - metabolism; Hepatocytes - microbiology; Humans; Listeria monocytogenes - genetics; Listeria monocytogenes - metabolism; Listeria monocytogenes - pathogenicity; Listeriosis - genetics; Listeriosis - metabolism; Listeriosis - microbiology; Membrane Proteins - genetics; Membrane Proteins - metabolism; Peptide Termination Factors - genetics; Peptide Termination Factors - metabolism; Virulence; host cell invasion; listeriosis; InlA; Listeria monocytogenes; pore-forming toxins; InlB; internalin; listeriolysin O
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/IAI.00555-18

is a facultative intracellular pathogen that infects a wide variety of cells, causing the life-threatening disease listeriosis. virulence factors include two surface invasins, InlA and InlB, known to promote bacterial uptake by host cells, and the secreted pore-forming toxin listeriolysin O (LLO), which disrupts the phagosome to allow bacterial proliferation in the cytosol. In addition, plasma membrane perforation by LLO has been shown to facilitate internalization into epithelial cells. In this work, we tested the host cell range and importance of LLO-mediated internalization relative to the canonical invasins, InlA and InlB. We measured the efficiencies of association with and internalization into several human cell types (hepatocytes, cytotrophoblasts, and endothelial cells) using wild-type bacteria and isogenic single, double, and triple deletion mutants for the genes encoding InlA, InlB and LLO. No role for InlB was detected in any tested cells unless the InlB expression level was substantially enhanced, which was achieved by introducing a mutation ( ) in the gene encoding the transcription factor PrfA. In contrast, InlA and LLO were the most critical invasion factors, although they act in a different manner and in a cell-type-dependent fashion. As expected, InlA facilitates both bacterial attachment and internalization in cells that express its receptor, E-cadherin. LLO promotes internalization into hepatocytes, but not into cytotrophoblasts and endothelial cells. Finally, LLO and InlA cooperate to increase the efficiency of host cell invasion by .